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Methods in Molecular Biology

Protein Acetylation

Methods and Protocols

Editors: Brosh Jr, Robert M. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key note and implementation advice from the experts
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eBook $169.00
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  • ISBN 978-1-4939-9434-2
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  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9433-5
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  • Usually dispatched within 3 to 5 business days.
About this book

The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems.



Table of contents (17 chapters)

Table of contents (17 chapters)
  • High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets

    Pages 3-16

    Schilling, Birgit (et al.)

  • Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry

    Pages 17-27

    Agudelo Garcia, Paula A. (et al.)

  • Detection and Quantitation of Acetylated Histones on Replicating DNA Using In Situ Proximity Ligation Assay and Click-It Chemistry

    Pages 29-45

    Lazarchuk, Pavlo (et al.)

  • Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC

    Pages 49-56

    Njeri, Catherine W. (et al.)

  • Measurement and Analysis of Lysine Acetylation by KAT Complexes In Vitro and In Vivo

    Pages 57-77

    Lashgari, Anahita (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9434-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9433-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Acetylation
Book Subtitle
Methods and Protocols
Editors
  • Robert M. Brosh Jr
Series Title
Methods in Molecular Biology
Series Volume
1983
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9434-2
DOI
10.1007/978-1-4939-9434-2
Hardcover ISBN
978-1-4939-9433-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 310
Number of Illustrations
12 b/w illustrations, 40 illustrations in colour
Topics