- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Buy this book
- About this book
-
This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic and applied researchers pursuing this vital area of research in pharmacology and toxicology.
- Table of contents (26 chapters)
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Mechanisms of Drug-Induced Cholestasis
Pages 1-14
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LC-MS/MS Analysis of Bile Acids in In Vitro Samples
Pages 15-23
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Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis
Pages 25-53
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Vesicle- and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function
Pages 55-73
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The C-DILI™ Assay: An Integrated In Vitro Approach to Predict Cholestatic Hepatotoxicity
Pages 75-85
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Experimental Cholestasis Research
- Editors
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- Mathieu Vinken
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1981
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9420-5
- DOI
- 10.1007/978-1-4939-9420-5
- Hardcover ISBN
- 978-1-4939-9419-9
- Softcover ISBN
- 978-1-4939-9422-9
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIII, 399
- Number of Illustrations
- 14 b/w illustrations, 60 illustrations in colour
- Topics
