Single Cell Methods
Sequencing and Proteomics
Editors: Proserpio, Valentina (Ed.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab. - About the authors
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- Table of contents (26 chapters)
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Setting Up a Single-Cell Genomic Laboratory
Pages 3-8
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Tissue Handling and Dissociation for Single-Cell RNA-Seq
Pages 9-21
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Full-Length Single-Cell RNA Sequencing with Smart-seq2
Pages 25-44
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CEL-Seq2—Single-Cell RNA Sequencing by Multiplexed Linear Amplification
Pages 45-56
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Single-Cell RNA-Seq by Multiple Annealing and Tailing-Based Quantitative Single-Cell RNA-Seq (MATQ-Seq)
Pages 57-71
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Single Cell Methods
- Book Subtitle
- Sequencing and Proteomics
- Editors
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- Valentina Proserpio
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1979
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9240-9
- DOI
- 10.1007/978-1-4939-9240-9
- Hardcover ISBN
- 978-1-4939-9239-3
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XVI, 459
- Number of Illustrations
- 9 b/w illustrations, 78 illustrations in colour
- Topics
