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Methods in Molecular Biology

Single Cell Methods

Sequencing and Proteomics

Editors: Proserpio, Valentina (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9240-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9239-3
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab.



   


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Table of contents (26 chapters)

Table of contents (26 chapters)
  • Setting Up a Single-Cell Genomic Laboratory

    Pages 3-8

    Mamanova, Lira

  • Tissue Handling and Dissociation for Single-Cell RNA-Seq

    Pages 9-21

    Vieira Braga, Felipe A. (et al.)

  • Full-Length Single-Cell RNA Sequencing with Smart-seq2

    Pages 25-44

    Picelli, Simone

  • CEL-Seq2—Single-Cell RNA Sequencing by Multiplexed Linear Amplification

    Pages 45-56

    Yanai, Itai (et al.)

  • Single-Cell RNA-Seq by Multiple Annealing and Tailing-Based Quantitative Single-Cell RNA-Seq (MATQ-Seq)

    Pages 57-71

    Sheng, Kuanwei (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9240-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9239-3
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Single Cell Methods
Book Subtitle
Sequencing and Proteomics
Editors
  • Valentina Proserpio
Series Title
Methods in Molecular Biology
Series Volume
1979
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9240-9
DOI
10.1007/978-1-4939-9240-9
Hardcover ISBN
978-1-4939-9239-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 459
Number of Illustrations
9 b/w illustrations, 78 illustrations in colour
Topics