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High-Throughput Metabolomics

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Angelo D'Alessandro

    1. Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1978)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xv
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  2. Methods

    1. Front Matter

      Pages 1-1
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    2. Sample Preparation and Reporting Standards for Metabolomics of Adherent Mammalian Cells

      • Sarah Hayton, Robert D. Trengove, Garth L. Maker
      Pages 3-12

    3. High-Throughput Metabolomics: Isocratic and Gradient Mass Spectrometry-Based Methods

      • Travis Nemkov, Julie A. Reisz, Sarah Gehrke, Kirk C. Hansen, Angelo D’Alessandro
      Pages 13-26

    4. High-Throughput Metabolomics Based on Direct Mass Spectrometry Analysis in Biomedical Research

      • Raúl González-Domínguez, Álvaro González-Domínguez, Carmen Segundo, Mónica Schwarz, Ana Sayago, Rosa María Mateos et al.
      Pages 27-38

    5. Traveling Wave Ion Mobility Mass Spectrometry: Metabolomics Applications

      • Giuseppe Paglia, Giuseppe Astarita
      Pages 39-53

    6. Capillary Electrophoresis Mass Spectrometry as a Tool for Untargeted Metabolomics

      • Ángeles López-Gonzálvez, Joanna Godzien, Antonia García, Coral Barbas
      Pages 55-77

  3. Lipidomics

    1. Front Matter

      Pages 79-79
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    2. Overview of Lipid Mass Spectrometry and Lipidomics

      • Simona Zarini, Robert M. Barkley, Miguel A. Gijón, Robert C. Murphy
      Pages 81-105

    3. LC-MS/MS-MRM-Based Targeted Metabolomics for Quantitative Analysis of Polyunsaturated Fatty Acids and Oxylipins

      • Xiaoyun Fu, Mikayla Anderson, Yi Wang, James C. Zimring
      Pages 107-120

    4. Untargeted and Semi-targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics

      • Julie A. Reisz, Connie Zheng, Angelo D’Alessandro, Travis Nemkov
      Pages 121-135

    5. HPLC-MS/MS Methods for Diacylglycerol and Sphingolipid Molecular Species in Skeletal Muscle

      • Kathleen A. Harrison, Bryan C. Bergman
      Pages 137-152

  4. Metabolomics of Animal and Plant Models

    1. Front Matter

      Pages 153-153
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    2. Quantification of d- and l-2-Hydroxyglutarate in Drosophila melanogaster Tissue Samples Using Gas Chromatography-Mass Spectrometry

      • Hongde Li, Jason M. Tennessen
      Pages 155-165

    3. Comprehensive LC-MS-Based Metabolite Fingerprinting Approach for Plant and Fungal-Derived Samples

      • Kirstin Feussner, Ivo Feussner
      Pages 167-185

    4. Untargeted Metabolomics of Plant Leaf Tissues

      • Federica Gevi, Giuseppina Fanelli, Lello Zolla, Sara Rinalducci
      Pages 187-195

  5. Tracing Experiments and Metabolic Flux Analysis

    1. Front Matter

      Pages 197-197
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    2. Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling

      • Gretchen L. Seim, Emily C. Britt, Jing Fan
      Pages 199-217

    3. Quantifying Intermediary Metabolism and Lipogenesis in Cultured Mammalian Cells Using Stable Isotope Tracing and Mass Spectrometry

      • Thekla Cordes, Christian M. Metallo
      Pages 219-241

    4. Insights into Dynamic Network States Using Metabolomic Data

      • Reihaneh Mostolizadeh, Andreas Dräger, Neema Jamshidi
      Pages 243-258
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Keywords

About this book

This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about thenext generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine.

Editors and Affiliations

  • Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA

    Angelo D'Alessandro

Bibliographic Information

  • Book Title: High-Throughput Metabolomics

  • Book Subtitle: Methods and Protocols

  • Editors: Angelo D'Alessandro

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9236-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9235-5Published: 23 May 2019

  • Softcover ISBN: 978-1-4939-9238-6Published: 26 September 2020

  • eBook ISBN: 978-1-4939-9236-2Published: 22 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 459

  • Number of Illustrations: 20 b/w illustrations, 63 illustrations in colour

  • Topics: Metabolomics, Human Genetics, Lipidology, Computational Biology/Bioinformatics

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