Methods in Molecular Biology

Mass Spectrometry of Proteins

Methods and Protocols

Editors: Evans, Caroline A., Wright, Phillip C., Noirel, Josselin (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
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  • ISBN 978-1-4939-9232-4
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Hardcover $199.99
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  • ISBN 978-1-4939-9231-7
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About this book

New insights into modern medicine and systems biology are enabled by innovative protocols and advanced technologies in mass spectrometry-based proteomics. This volume details new pipelines, workflows, and ways to process data that allow for new frontiers in proteomics to be pushed forward. With applications to biomarker discovery, interactions between proteins, between biological systems, dynamics of post-translational modifications among others, new protocols have been developed and iteratively refined to probe the endless complexity of the proteome in ever greater details. This volume deals with methods for data dependent and data independent mass spectrometry analyses. Valuable, first-hand information is provided from designing experiments, sample preparation and analysis, exploitation of public datasets and carrying out reproducible data pipelines, using modern computational tools such as Galaxy or Jupyter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Mass Spectrometry of Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field. 


Table of contents (16 chapters)

Table of contents (16 chapters)
  • Next Generation Proteomics for Clinical Biomarker Detection Using SWATH-MS

    Lin, Qifeng (et al.)

    Pages 3-15

  • A Combined Chemical Derivatization/Mass Spectrometric Method for the Enhanced Detection and Relative Quantification of Protein Ubiquitination

    Chicooree, Navin (et al.)

    Pages 17-24

  • Assessment of Ubiquitin Chain Topology by Targeted Mass Spectrometry

    Longworth, Joseph (et al.)

    Pages 25-34

  • Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation

    Barrios-Llerena, Martin E. (et al.)

    Pages 35-42

  • Isotopic Labeling and Quantitative Proteomics of Acetylation on Histones and Beyond

    Lund, Peder J. (et al.)

    Pages 43-70

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9232-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9231-7
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Mass Spectrometry of Proteins
Book Subtitle
Methods and Protocols
Editors
  • Caroline A. Evans
  • Phillip C. Wright
  • Josselin Noirel
Series Title
Methods in Molecular Biology
Series Volume
1977
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9232-4
DOI
10.1007/978-1-4939-9232-4
Hardcover ISBN
978-1-4939-9231-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 264
Number of Illustrations
19 b/w illustrations, 35 illustrations in colour
Topics