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RNA Interference and Cancer Therapy

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Lekha Dinesh Kumar

    1. Cancer Biology, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1974)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
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  2. siRNA Library Screening to Identify Complementary Therapeutic Pairs in Triple-Negative Breast Cancer Cells

    • Bindu Thapa, KC Remant, Hasan Uludağ
    Pages 1-19

  3. In Vitro Evaluation of Candidate Gene Targets for Cancer Therapy

    • Xing Fei Tan, Wei Xuan Teo, George W. Yip
    Pages 21-30

  4. siRNA-Based Drug Targeting Human Bcl-xL Against Cancers

    • Yoshifumi Takei
    Pages 31-40

  5. Relative Quantification of siRNA Strand Loading into Ago2 for Design of Highly Active siRNAs

    • Phillip A. Angart, Kwasi Adu-Berchie, Rebecca J. Carlson, Daniel B. Vocelle, Christina Chan, S. Patrick Walton
    Pages 41-56

  6. VEGFA Gene Silencing in CXCR4-Expressing Cells via siRNA Delivery by Means of Targeted Peptide Carrier

    • Anna A. Egorova, Marianna A. Maretina, Anton V. Kiselev
    Pages 57-68

  7. Design Strategy to Access siRNA-Encapsulating DNA “Nanosuitcases” That Can Conditionally Release Their Cargo

    • Katherine E. Bujold, Hassan H. Fakih, Hanadi F. Sleiman
    Pages 69-81

  8. Combinatorial siRNA Polyplexes for Receptor Targeting

    • Dian-Jang Lee, Ernst Wagner
    Pages 83-98

  9. Synthesis of Doxorubicin and miRNA Stimuli-Sensitive Conjugates for Combination Therapy

    • Daniel F. Costa, Can Sarisozen, Vladimir P. Torchilin
    Pages 99-109

  10. Minicell-Based Targeted Delivery of shRNA to Cancer Cells: An Experimental Protocol

    • Mehul Jivrajani, Manish Nivsarkar
    Pages 111-139

  11. Targeted Delivery of miRNA Antagonists to Myeloid Cells In Vitro and In Vivo

    • Yu-Lin Su, Piotr Swiderski, Guido Marcucci, Marcin Kortylewski
    Pages 141-150

  12. Systemic Modulation of Lymphocyte Subsets Using siRNAs Delivered via Targeted Lipid Nanoparticles

    • Inbal Hazan-Halevy, Daniel Rosenblum, Srinivas Ramishetti, Dan Peer
    Pages 151-159

  13. Targeting Cancer with Peptide RNAi Nanoplexes

    • A. James Mixson, Qixin Leng, Szu-Ting Chou, Martin C. Woodle
    Pages 161-180

  14. Preparation of a Carrier to Achieve In Vivo Delivery of siRNA: The Example of Chitosan-Coated Poly(isobutylcyanoacrylate) Nanoparticles

    • Christine Vauthier
    Pages 181-194

  15. Monoclonal Antibody-Conjugated Dendritic Nanostructures for siRNA Delivery

    • Hitesh Kulhari, Ashok K. Jangid, David J. Adams
    Pages 195-201

  16. Synthesis of Gold Nanoparticles for Gene Silencing

    • Claudia Tortiglione, Jesús M de la Fuente
    Pages 203-214

  17. Design of Eco-Friendly Gold Nanoparticles for Cancer Treatment

    • Deep Pooja, Ramakrishna Sistla
    Pages 215-221

  18. 64Cu-Labeled Aptamers for Tumor-Targeted Radionuclide Delivery

    • Lei Kang, Zachary T. Rosenkrans, Weibo Cai
    Pages 223-231

  19. Silencing PRDM14 via Oligonucleotide Therapeutics Suppresses Tumorigenicity and Metastasis of Breast Cancer

    • Hiroaki Taniguchi, Kohzoh Imai
    Pages 233-243

  20. Biodrug Suppresses Breast and Colorectal Cancer in Murine Models

    • Syed Sultan Beevi, Naveen Kumar Tangudu, Vinod Kumar Verma, Lekha Dinesh Kumar
    Pages 245-263
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Keywords

About this book

This comprehensive volume collects a repertoire of techniques for the adoption and exploitation of RNA interference (RNAi) as a fertile strategy to develop the bio-drugs of future in the vital field of cancer research. Exploring the immense ‘bench to bedside’ potential of RNAi as a therapeutic molecule, the book focuses many of its chapters on nanoparticle-based targeted deliveries, including aptamers, as well as techniques for using RNAi technology to combat various cancers in preclinical studies. Each of the 26 chapters is authored by the pioneers in the respective areas and thus provides a practical and empirical account of the techniques that could be used as benchmark tools in the area of cancer research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and essential, RNA Interference and Cancer Therapy: Methods and Protocols provides the necessary guidance for both novice and professional researchers who intend to discover and innovate newer means of using RNAi technology to combat cancer, one of the greatest scourges of human health.

Editors and Affiliations

  • Cancer Biology, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India

    Lekha Dinesh Kumar

Bibliographic Information

  • Book Title: RNA Interference and Cancer Therapy

  • Book Subtitle: Methods and Protocols

  • Editors: Lekha Dinesh Kumar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9220-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9219-5Published: 17 May 2019

  • Softcover ISBN: 978-1-4939-9222-5Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9220-1Published: 16 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 416

  • Number of Illustrations: 28 b/w illustrations, 84 illustrations in colour

  • Topics: Cancer Research, Human Genetics, Pharmaceutical Sciences/Technology

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