Methods in Molecular Biology

Neisseria meningitidis

Methods and Protocols

Editors: Seib, Kate L., Peak, Ian R. (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains implementation advice from the experts
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About this book

This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration and consistency in investigations of this enigmatic and dangerous pathogen.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • An Overview of Neisseria meningitidis

    Hollingshead, Sarah (et al.)

    Pages 1-16

  • Diagnosis of Meningococcal Infection Using Internally Controlled Multiplex Real-Time PCR

    Deghmane, Ala-Eddine (et al.)

    Pages 17-31

  • Investigating RNA–Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis

    Heidrich, Nadja (et al.)

    Pages 33-49

  • Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics

    Bratcher, Holly B. (et al.)

    Pages 51-82

  • Determination of Repeat Number and Expression States of Phase-Variable Loci Through Next Generation Sequencing and Bioinformatic Analysis

    Green, Luke R. (et al.)

    Pages 83-92

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9202-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9201-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Neisseria meningitidis
Book Subtitle
Methods and Protocols
Editors
  • Kate L. Seib
  • Ian R. Peak
Series Title
Methods in Molecular Biology
Series Volume
1969
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9202-7
DOI
10.1007/978-1-4939-9202-7
Hardcover ISBN
978-1-4939-9201-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 253
Number of Illustrations
16 b/w illustrations, 42 illustrations in colour
Topics