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Methods in Molecular Biology

Nuclear Receptors

Methods and Experimental Protocols

Editors: Badr, Mostafa Z. (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts 
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eBook $149.00
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  • ISBN 978-1-4939-9195-2
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About this book

This volume presents valuable techniques for studying the class of ligand-activated transcription factors known as nuclear receptors. After a brief overview of the history of the field, chapters cover methods to detect the receptors and their mRNAs in various tissues, protocols to characterize nuclear receptor modulators and activities, their signaling and roles in certain pathogenesis, molecular modeling of nuclear receptor-ligand interactions, as well as the utility of informatics in the field of nuclear receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and cutting-edge, Nuclear Receptors: Methods and Experimental Protocols seeks to aid researchers working toward furthering our understanding of these vital receptors and their role in numerous pathological conditions.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • Nuclear Receptors: A Historical Perspective

    Pages 1-5

    Mazaira, Gisela I. (et al.)

  • An Optimized Immunoblotting Protocol for Accurate Detection of Endogenous PGC-1α Isoforms in Various Rodent Tissues

    Pages 7-16

    Gettys, Thomas W. (et al.)

  • Subcellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue

    Pages 17-26

    Smyth, Aisling (et al.)

  • In Vivo Quantitation of Estrogen Receptor β Subtype Expression in Ovarian Surface Epithelium Using Immunofluorescence Profiling and Confocal Microscopy

    Pages 27-38

    Gulliver, Linda S. M.

  • In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High-Confidence Genome-Wide Binding Profiles

    Pages 39-70

    Mir, Ashfaq Ali (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-4939-9195-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Nuclear Receptors
Book Subtitle
Methods and Experimental Protocols
Editors
  • Mostafa Z. Badr
Series Title
Methods in Molecular Biology
Series Volume
1966
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9195-2
DOI
10.1007/978-1-4939-9195-2
Hardcover ISBN
978-1-4939-9194-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 314
Number of Illustrations
15 b/w illustrations, 57 illustrations in colour
Topics