Methods in Molecular Biology

Ancient DNA

Methods and Protocols

Editors: Shapiro, B., Barlow, A., Heintzman, P.D., Hofreiter, M., Paijmans, J.L.A., Soares, A.E.R. (Eds.)

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  • Includes cutting-edge, fully updated techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $89.00
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  • ISBN 978-1-4939-9176-1
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Hardcover $119.99
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About this book

This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. The volume also examines the analysis of high-throughput sequencing data recovered from ancient specimens, which, because of the degraded nature of ancient DNA and common co-extraction of contaminant DNA, has challenges that are unique compared to data recovered from modern specimens.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and cutting-edge, Ancient DNA: Methods and Protocols, Second Edition aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab.

Table of contents (18 chapters)

Table of contents (18 chapters)
  • Setting Up an Ancient DNA Laboratory

    Fulton, Tara L. (et al.)

    Pages 1-13

  • Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate

    Korlević, Petra (et al.)

    Pages 15-19

  • Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Predigestion Step

    Schroeder, Hannes (et al.)

    Pages 21-24

  • Extraction of Highly Degraded DNA from Ancient Bones and Teeth

    Dabney, Jesse (et al.)

    Pages 25-29

  • Sampling and Extraction of Ancient DNA from Sediments

    Epp, Laura S. (et al.)

    Pages 31-44

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9176-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.99
price for USA in USD
  • ISBN 978-1-4939-9175-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Ancient DNA
Book Subtitle
Methods and Protocols
Editors
  • Beth Shapiro
  • Axel Barlow
  • Peter D. Heintzman
  • Michael Hofreiter
  • Johanna L. A. Paijmans
  • André E. R. Soares
Series Title
Methods in Molecular Biology
Series Volume
1963
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9176-1
DOI
10.1007/978-1-4939-9176-1
Hardcover ISBN
978-1-4939-9175-4
Series ISSN
1064-3745
Edition Number
2
Number of Pages
X, 216
Number of Illustrations
10 b/w illustrations, 5 illustrations in colour
Topics