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Methods in Molecular Biology

CRISPR Gene Editing

Methods and Protocols

Editors: Luo, Yonglun (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $169.00
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  • ISBN 978-1-4939-9170-9
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  • Included format: PDF, EPUB
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Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9169-3
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  • Usually dispatched within 3 to 5 business days.
About this book

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field. 
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

Table of contents (21 chapters)

Table of contents (21 chapters)
  • CRISPR-gRNA Design

    Pages 3-11

    Pallarès Masmitjà, Maria (et al.)

  • Tracking CRISPR’s Footprints

    Pages 13-28

    Lin, Lin (et al.)

  • Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER

    Pages 29-44

    Brinkman, Eva Karina (et al.)

  • Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA

    Pages 45-66

    König, Saskia (et al.)

  • Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check

    Pages 67-77

    Lin, Lin (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9170-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9169-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
CRISPR Gene Editing
Book Subtitle
Methods and Protocols
Editors
  • Yonglun Luo
Series Title
Methods in Molecular Biology
Series Volume
1961
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9170-9
DOI
10.1007/978-1-4939-9170-9
Hardcover ISBN
978-1-4939-9169-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 362
Number of Illustrations
15 b/w illustrations, 61 illustrations in colour
Topics