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Mouse Models of Innate Immunity

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Irving C. Allen

    1. Department of Biomedical Sciences, Virginia-Maryland Regional College, Blacksburg, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1960)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. CRISPR/Cas9-Assisted Genome Editing in Murine Embryonic Stem Cells

    • Artiom Gruzdev, Greg J. Scott, Thomas B. Hagler, Manas K. Ray
    Pages 1-21

  3. Genome Editing in Mouse Embryos with CRISPR/Cas9

    • Greg J. Scott, Artiom Gruzdev
    Pages 23-40

  4. Derivation of Macrophages from Mouse Bone Marrow

    • Beckley K. Davis
    Pages 41-55

  5. Bone Marrow-Derived Dendritic Cells

    • Kelly Roney
    Pages 57-62

  6. Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils

    • Linda Vong, Philip M. Sherman, Michael Glogauer
    Pages 63-73

  7. In Vitro Differentiation of Effector CD4+ T Helper Cell Subsets

    • Kaitlin A. Read, Michael D. Powell, Bharath K. Sreekumar, Kenneth J. Oestreich
    Pages 75-84

  8. Generation and Culture of Mouse Embryonic Fibroblasts

    • Yee Sun Tan, Yu L. Lei
    Pages 85-91

  9. Isolation of Tumor-Infiltrating Lymphocytes by Ficoll-Paque Density Gradient Centrifugation

    • Yee Sun Tan, Yu L. Lei
    Pages 93-99

  10. Depletion and Reconstitution of Macrophages in Mice

    • Lisa K. Kozicky, Laura M. Sly
    Pages 101-112

  11. Microfluidic Platform to Quantify Neutrophil Migratory Decision-Making

    • Brittany P. Boribong, Amina Rahimi, Caroline N. Jones
    Pages 113-122

  12. A Vector Suite for the Overexpression and Purification of Tagged Outer Membrane, Periplasmic, and Secreted Proteins in E. coli

    • Michael A. Casasanta, Daniel J. Slade
    Pages 123-138

  13. Mouse Model of Staphylococcus aureus Skin Infection

    • Natalia Malachowa, Scott D. Kobayashi, Jamie Lovaglio, Frank R. DeLeo
    Pages 139-147

  14. Systemic Listeria monocytogenes Infection as a Model to Study T Helper Cell Immune Responses

    • Veronica M. Ringel-Scaia, Michael D. Powell, Kaitlin A. Read, Irving C. Allen, Kenneth J. Oestreich
    Pages 149-160

  15. Endotoxin-Induced Uveitis in Rodents

    • Umesh C. S. Yadav, Kota V. Ramana
    Pages 161-168

  16. Using Klebsiella pneumoniae to Model Acute Lung Inflammation in Mice

    • Dylan K. McDaniel, Irving C. Allen
    Pages 169-180

  17. Assessment of Survival and Replication of Brucella spp. in Murine Peritoneal Macrophages

    • Clayton C. Caswell
    Pages 181-189

  18. Influenza-Mediated Lung Infection Models

    • Charles E. McGee, Christopher J. Sample, Brita Kilburg-Basnyat, Kristin A. Gabor, Michael B. Fessler, Kymberly M. Gowdy
    Pages 191-205

  19. Adoptive Transfer Colitis

    • Kristin Eden
    Pages 207-214

  20. The Azoxymethane/Il10 −/− Model of Colitis-Associated Cancer (CAC)

    • Aaron Rothemich, Janelle C. Arthur
    Pages 215-225
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Keywords

About this book

This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function. New and updated chapters detail protocols on in vitro and ex vivo studies in key cell types associated with innate immunity and with in vivo protocols used to study immune system function in the mouse. Additionally, chapters describe methods to evaluate innate immune function and new protocols associated with autism, cancer, microfluidics platforms, and CRISPR systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols, Second Edition will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune system.


Editors and Affiliations

  • Department of Biomedical Sciences, Virginia-Maryland Regional College, Blacksburg, USA

    Irving C. Allen

Bibliographic Information

  • Book Title: Mouse Models of Innate Immunity

  • Book Subtitle: Methods and Protocols

  • Editors: Irving C. Allen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9167-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9166-2Published: 24 February 2019

  • eBook ISBN: 978-1-4939-9167-9Published: 23 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 258

  • Topics: Immunology, Animal Models

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