Methods in Molecular Biology

Proteomics for Biomarker Discovery

Methods and Protocols

Editors: Brun, Virginie, Couté, Yohann (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains implementation advice from the experts
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  • ISBN 978-1-4939-9164-8
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Hardcover $199.99
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About this book

This volume presents modern and enhanced methods that detail techniques to perform proteomics analyses dedicated to biomarker discovery for human health. Chapters guide readers through pre/post analytical factors, protocols for the preparation of extracellular vesicles and exosomes, and various analytical pipelines including Data Independent Acquisition (DIA), discovery, as well as targeted and top-down proteomics analysis workflows. Bioinformatics tools and workflows to select and evaluate candidate biomarkers or combinations of biomarkers are also presented. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and cutting-edge, Proteomics for Biomarker Discovery: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Table of contents (18 chapters)

Table of contents (18 chapters)
  • Pre- and Post-analytical Factors in Biomarker Discovery

    Klont, Frank (et al.)

    Pages 1-22

  • Pre-fractionation of Noncirculating Biological Fluids to Improve Discovery of Clinically Relevant Protein Biomarkers

    Farina, Annarita

    Pages 23-37

  • Serum Exosome Isolation by Size-Exclusion Chromatography for the Discovery and Validation of Preeclampsia-Associated Biomarkers

    Navajas, Rosana (et al.)

    Pages 39-50

  • Protein Biomarker Discovery Using Human Blood Plasma Microparticles

    Taleb, Raghda Saad Zaghloul (et al.)

    Pages 51-64

  • A Standardized and Reproducible Proteomics Protocol for Bottom-Up Quantitative Analysis of Protein Samples Using SP3 and Mass Spectrometry

    Hughes, Christopher S. (et al.)

    Pages 65-87

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9164-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9163-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Proteomics for Biomarker Discovery
Book Subtitle
Methods and Protocols
Editors
  • Virginie Brun
  • Yohann Couté
Series Title
Methods in Molecular Biology
Series Volume
1959
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9164-8
DOI
10.1007/978-1-4939-9164-8
Hardcover ISBN
978-1-4939-9163-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 292
Number of Illustrations
5 b/w illustrations, 47 illustrations in colour
Topics