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Beta-Arrestins

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Mark G. H. Scott

    1. Institut Cochin, INSERM U1016, Paris, France

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  2. Stéphane A. Laporte

    1. Department of Medicine, Research Institute of the McGill, University Health Center (RI-MUHC), McGill University, Montreal, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1957)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. A Brief History of the β-Arrestins

      • Marc G. Caron, Lawrence S. Barak
      Pages 3-8

    3. β-Arrestins: Multitask Scaffolds Orchestrating the Where and When in Cell Signalling

      • Stéphane A. Laporte, Mark G. H. Scott
      Pages 9-55

  4. Methods to Study Scaffolding Functions of β-Arrestins

    1. Front Matter

      Pages 105-105
      Download chapter PDF

    2. Using In Vitro Pull-Down and In-Cell Overexpression Assays to Study Protein Interactions with Arrestin

      • Nicole A. Perry, Xuanzhi Zhan, Eugenia V. Gurevich, T. M. Iverson, Vsevolod V. Gurevich
      Pages 107-120

    3. Methods to Investigate Arrestins in Complex with Phosphodiesterases

      • Amy J. Tibbo, Tara Busiau, George S. Baillie
      Pages 121-137

    4. Methods to Characterize Protein Interactions with β-Arrestin In Cellulo

      • Revu Ann Alexander, Isaure Lot, Hervé Enslen
      Pages 139-158

    5. Methods to Investigate the β-Arrestin-Mediated Control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling

      • Ricardo Charles, Mohamed Bourmoum, Shirley Campbell, Audrey Claing
      Pages 159-168

    6. Methods to Investigate the Roles of β-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing

      • Stephen S. G. Ferguson
      Pages 169-175

    7. Methods to Determine Interaction Interfaces Between β-Arrestins and Their Protein Partners

      • Thomas Bourquard, Astrid Musnier, Aurélie Tréfier, Flavie Landomiel, Thomas Boulo, Eric Reiter et al.
      Pages 177-194

    8. Workflow Description to Dynamically Model β-Arrestin Signaling Networks

      • Romain Yvinec, Mohammed Akli Ayoub, Francesco De Pascali, Pascale Crépieux, Eric Reiter, Anne Poupon
      Pages 195-215

    9. Proteomic Analysis of the β-Arrestin Interactomes

      • Yang Zhao, Kunhong Xiao
      Pages 217-232

  5. Methods to Analyse Ligand Bias

    1. Front Matter

      Pages 233-233
      Download chapter PDF

    2. Quantitating Ligand Bias Using the Competitive Model of Ligand Activity

      • Edward L. Stahl, Frederick J. Ehlert, Laura M. Bohn
      Pages 235-247
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Keywords

About this book

This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in  MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson’s disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents,step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins.


Editors and Affiliations

  • Institut Cochin, INSERM U1016, Paris, France

    Mark G. H. Scott

  • Department of Medicine, Research Institute of the McGill, University Health Center (RI-MUHC), McGill University, Montreal, Canada

    Stéphane A. Laporte

Bibliographic Information

  • Book Title: Beta-Arrestins

  • Book Subtitle: Methods and Protocols

  • Editors: Mark G. H. Scott, Stéphane A. Laporte

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9158-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9157-0Published: 28 March 2019

  • eBook ISBN: 978-1-4939-9158-7Published: 27 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 409

  • Number of Illustrations: 19 b/w illustrations, 60 illustrations in colour

  • Topics: Protein Science, Cell Biology, Receptors

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