Skip to main content
SpringerLink
Log in

Bacterial Polysaccharides

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Inka Brockhausen

    1. Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1954)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Customizable Cloning of Whole Polysaccharide Gene Clusters by Yeast Homologous Recombination

    • Michael A. Liu, Peter R. Reeves
    Pages 1-14

  3. RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-Negative Bacteria

    • Zhongrui Ma, Peng George Wang
    Pages 15-23

  4. Purification of Capsular Polysaccharide Complex from Gram-Negative Bacteria

    • Carlos A. Alvarez, Brian A. Cobb
    Pages 25-35

  5. Simple Protocol to Purify Cell Wall Polysaccharide from Gram-Positive Bacteria and Assess Its Structural Integrity

    • Irina Sadovskaya, Yann Guérardel
    Pages 37-45

  6. Extraction and Purification of Wall-Bound Polymers of Gram-Positive Bacteria

    • Alice Chateau, Olaf Schneewind, Dominique Missiakas
    Pages 47-57

  7. Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan, and Lipoarabinomannan

    • Kathryn C. Rahlwes, Julia Puffal, Yasu S. Morita
    Pages 59-75

  8. Conjugation of Synthetic Oligosaccharides to Proteins by Squaric Acid Chemistry

    • Hélène B. Pfister, Xiaowei Lu, Sameh E. Soliman, Pavol Kováč
    Pages 77-88

  9. Direct Conjugation of Bacterial Polysaccharides to Proteins by Squaric Acid Chemistry

    • Peng Xu, Pavol Kováč
    Pages 89-98

  10. Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from Streptococcus equi for Studies in Cytokine Production

    • Siziwe Bebe, Tassos Anastassiades
    Pages 99-113

  11. Assays for the Enzymes Catalyzing the O-Acetylation of Bacterial Cell Wall Polysaccharides

    • Ashley S. Brott, David Sychantha, Anthony J. Clarke
    Pages 115-136

  12. Designing Glycosyltransferase Expression Constructs for Improved Purification, Protein Yield, and Crystallization

    • Taylor J. B. Forrester, Lana El Osta, Joseph S. Lam, Matthew S. Kimber
    Pages 137-150

  13. Utilization of Fluorescently Tagged Synthetic Acceptor Molecules for In Vitro Characterization of a Dual-Domain Glycosyltransferase Enzyme, KpsC, from Escherichia coli

    • Liam Doyle, Olga G. Ovchinnikova, Chris Whitfield
    Pages 151-159

  14. Synthesis of Phenoxyundecyl Diphosphate Disaccharides for Studies of the Biosynthesis of O Antigenic Polysaccharides in Enteric Bacteria

    • Vladimir Torgov, Leonid Danilov, Natalia Utkina, Vladimir Veselovsky, Alexander Kocev, Inka Brockhausen
    Pages 161-174

  15. Characterization of Arabinosyl Transfer Reactions in the Biosynthesis of Mycobacterial Cell Envelope (Lipo)Polysaccharides

    • Shiva Kumar Angala, Mary Jackson
    Pages 175-186

  16. Enzymatic Synthesis of Repeating Unit Oligosaccharides of Escherichia coli O104

    • Inka Brockhausen, Diana Czuchry
    Pages 187-202

  17. Sulfhydryl Labeling as a Tool to Investigate the Topology of Membrane Proteins Involved in Lipopolysaccharide Biosynthesis

    • Faviola Tavares-Carreón, Xiang Ruan, Amy Ford, Miguel A. Valvano
    Pages 203-213

  18. Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides

    • Tasnim Abukar, Nakita Buenbrazo, Bettina Janesch, Laura Kell, Warren Wakarchuk
    Pages 215-235

  19. Glycosyltransferase Activity Assay Using Colorimetric Methods

    • M. Shafiqur Rahman, Wensheng Qin
    Pages 237-243

  20. In Vitro Characterization of a Multidomain Glycosyltransferase Using Fluorescently Tagged Synthetic Acceptors

    • Danielle M. Williams, Olga G. Ovchinnikova, Chris Whitfield
    Pages 245-253
Back to top

Keywords

About this book

This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, with their functions in bacterial adhesion, colonization, growth, establishment of biofilms, and control virulence and pathogenicity, are increasingly important. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Polysaccharides: Methods and Protocols aims to support researchers contributing to future approaches that will fill our knowledge gaps and define anti-bacterial targets.

Editors and Affiliations

  • Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, Canada

    Inka Brockhausen

Bibliographic Information

  • Book Title: Bacterial Polysaccharides

  • Book Subtitle: Methods and Protocols

  • Editors: Inka Brockhausen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9154-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9153-2Published: 13 March 2019

  • eBook ISBN: 978-1-4939-9154-9Published: 12 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 312

  • Number of Illustrations: 29 b/w illustrations, 41 illustrations in colour

  • Topics: Bacteriology, Enzymology, Immunology

Publish with us

Policies and ethics

Back to top

Search

Navigation