Methods in Molecular Biology

Bacterial Polysaccharides

Methods and Protocols

Editors: Brockhausen, Inka (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $169.00
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  • ISBN 978-1-4939-9154-9
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Hardcover $219.99
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About this book

This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, with their functions in bacterial adhesion, colonization, growth, establishment of biofilms, and control virulence and pathogenicity, are increasingly important. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Polysaccharides: Methods and Protocols aims to support researchers contributing to future approaches that will fill our knowledge gaps and define anti-bacterial targets.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • Customizable Cloning of Whole Polysaccharide Gene Clusters by Yeast Homologous Recombination

    Liu, Michael A. (et al.)

    Pages 1-14

  • RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-Negative Bacteria

    Ma, Zhongrui (et al.)

    Pages 15-23

  • Purification of Capsular Polysaccharide Complex from Gram-Negative Bacteria

    Alvarez, Carlos A. (et al.)

    Pages 25-35

  • Simple Protocol to Purify Cell Wall Polysaccharide from Gram-Positive Bacteria and Assess Its Structural Integrity

    Sadovskaya, Irina (et al.)

    Pages 37-45

  • Extraction and Purification of Wall-Bound Polymers of Gram-Positive Bacteria

    Chateau, Alice (et al.)

    Pages 47-57

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9154-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9153-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Bacterial Polysaccharides
Book Subtitle
Methods and Protocols
Editors
  • Inka Brockhausen
Series Title
Methods in Molecular Biology
Series Volume
1954
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9154-9
DOI
10.1007/978-1-4939-9154-9
Hardcover ISBN
978-1-4939-9153-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 312
Number of Illustrations
29 b/w illustrations, 41 illustrations in colour
Topics