Overview
- Editors:
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Karina Andrea Gómez
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INGEBI-CONICET, Buenos Aires, Argentina
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Carlos Andrés Buscaglia
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UNSAM-CONICET, IIB-INTECH, San Martin, Argentina
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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Table of contents (28 protocols)
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Front Matter
Pages i-xvii
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- João Luís Reis-Cunha, Daniella C. Bartholomeu
Pages 1-22
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- Christian Teh-Poot, Eric Dumonteil
Pages 23-34
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- Gonzalo Greif, Luisa Berná, Florencia Díaz-Viraqué, Carlos Robello
Pages 35-45
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- Alicia Majeau, Claudia Herrera, Eric Dumonteil
Pages 47-60
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- Noelia Lander, Miguel A. Chiurillo, Roberto Docampo
Pages 61-76
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- Carlos André Ornelas Ricart, Rayner Myr Lauterjung Queiroz, Agenor de Castro Moreira dos Santos Júnior, Sébastien Charneau
Pages 77-87
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- Izadora Volpato Rossi, Bruno Gavinho, Marcel Ivan Ramirez
Pages 89-104
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- María José Ferrer, Diana Patricia Wehrendt, Mariana Bonilla, Marcelo Alberto Comini, María Teresa Tellez-Iñón, Mariana Potenza
Pages 105-118
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- Virginia Balouz, Andrea C. Mesias, Camila Centeno Camean, Ivana Ducrey, Maite Mabel Lobo, Ignacio M. Durante et al.
Pages 119-134
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- Giannina Carlevaro, Andrés B. Lantos, Gaspar E. Cánepa, María de los Milagros Cámara, Martín Somoza, Carlos A. Buscaglia et al.
Pages 135-146
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- Martin C. Taylor, Amanda F. Francisco, Shiromani Jayawardhana, Gurdip Singh Mann, Alexander I. Ward, Francisco Olmo et al.
Pages 147-163
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- Matías Exequiel Rodríguez, Mariana Rizzi, Lucas Caeiro, Yamil Masip, Daniel O. Sánchez, Valeria Tekiel
Pages 165-177
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- Fernando Sánchez-Valdéz, Angel Padilla
Pages 179-186
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- Julien Santi-Rocca, Núria Gironès, Manuel Fresno
Pages 187-202
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- Ludmila Rodrigues Pinto Ferreira
Pages 203-214
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- Juan Carlos Ramírez, Otacilio da Cruz Moreira
Pages 215-225
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- Otacilio da Cruz Moreira, Juan Carlos Ramirez
Pages 227-238
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- Gerd Wallukat, Katrin Wenzel, Ingolf Schimke
Pages 247-261
About this book
This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput ‘-omics’ technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information.
Reviews
“The book is written for students, active researchers, and clinicians. … This book comprehensively covers the experimental methods for the study of the biology of T. cruzi and its complex interactions with the human host. The unique feature of the book is that the variety of methods and protocols it covers. There are very few books that provide the depth of coverage this book does.” (Omer Iqbal, Doody's Book Reviews, August 2019)
Editors and Affiliations
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INGEBI-CONICET, Buenos Aires, Argentina
Karina Andrea Gómez
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UNSAM-CONICET, IIB-INTECH, San Martin, Argentina
Carlos Andrés Buscaglia
