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Methods in Molecular Biology

T. cruzi Infection

Methods and Protocols

Editors: Gomez, Karina Andrea, Buscaglia, Carlos Andres (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9148-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9147-1
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput ‘-omics’ technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information.   

Reviews

“The book is written for students, active researchers, and clinicians. … This book comprehensively covers the experimental methods for the study of the biology of T. cruzi and its complex interactions with the human host. The unique feature of the book is that the variety of methods and protocols it covers. There are very few books that provide the depth of coverage this book does.” (Omer Iqbal, Doody's Book Reviews, August 2019)

Table of contents (28 chapters)

Table of contents (28 chapters)
  • Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex Genome

    Pages 1-22

    Reis-Cunha, João Luís (et al.)

  • Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine Development

    Pages 23-34

    Teh-Poot, Christian (et al.)

  • Transcriptome Studies in Trypanosoma cruzi Using RNA-seq

    Pages 35-45

    Greif, Gonzalo (et al.)

  • An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-exon Gene

    Pages 47-60

    Majeau, Alicia (et al.)

  • Genome Editing by CRISPR/Cas9 in Trypanosoma cruzi

    Pages 61-76

    Lander, Noelia (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9148-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9147-1
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
T. cruzi Infection
Book Subtitle
Methods and Protocols
Editors
  • Karina Andrea Gomez
  • Carlos Andres Buscaglia
Series Title
Methods in Molecular Biology
Series Volume
1955
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9148-8
DOI
10.1007/978-1-4939-9148-8
Hardcover ISBN
978-1-4939-9147-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVII, 398
Number of Illustrations
13 b/w illustrations, 47 illustrations in colour
Topics