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Methods in Molecular Biology

Adeno-Associated Virus Vectors

Design and Delivery

Editors: Castle, Michael J. (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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  • ISBN 978-1-4939-9139-6
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About this book

This volume provides a complete and timely guide to the use of adeno-associated virus (AAV)  vectors for genetic manipulation of mammalian tissues. Beginning with methods for the design and characterization of AAV vectors, the book continues with protocols for AAV delivery to various components of the central nervous system, to a number of sensory systems, and to a broad range of other tissues. Novel techniques such as ultrasound-targeted delivery to the brain, subpial delivery to the spinal cord, and subILM delivery to the retina are accompanied by chapters that provide an overview and comparison of current methods for AAV delivery to tissues such as brain, heart, liver, and lung. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. 
Authoritative and comprehensive, Adeno-Associated Virus Vectors: Design and Delivery aims to enhance the utility of AAV vectors for targeted gene transfer to living animals and continue the ongoing development of novel AAV-based gene therapies for human disease.

Table of contents (24 chapters)

Table of contents (24 chapters)
  • Design of AAV Vectors for Delivery of RNAi

    Pages 3-18

    Borel, Florie (et al.)

  • Design of AAV Vectors for Delivery of Large or Multiple Transgenes

    Pages 19-33

    Patel, Aman (et al.)

  • Ligand Coupling to the AAV Capsid for Cell-Specific Gene Transfer

    Pages 35-50

    Reul, Johanna (et al.)

  • Quantitative and Digital Droplet-Based AAV Genome Titration

    Pages 51-83

    Sanmiguel, Julio (et al.)

  • Single-Stranded DNA Virus Sequencing (SSV-Seq) for Characterization of Residual DNA and AAV Vector Genomes

    Pages 85-106

    Lecomte, Emilie (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-4939-9139-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Adeno-Associated Virus Vectors
Book Subtitle
Design and Delivery
Editors
  • Michael J. Castle
Series Title
Methods in Molecular Biology
Series Volume
1950
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9139-6
DOI
10.1007/978-1-4939-9139-6
Hardcover ISBN
978-1-4939-9138-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 426
Number of Illustrations
12 b/w illustrations, 50 illustrations in colour
Topics

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