Methods in Molecular Biology

Intracellular Lipid Transport

Methods and Protocols

Editors: Drin, Guillaume (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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Hardcover $219.99
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About this book

This detailed book provides technical approaches to tackle a variety of questions related to intracellular lipid transport in order to improve our understanding at different scales of how lipids are accurately displaced between organelles, across long distances or at membrane contact sites, or within cellular membranes. The volume begins with methodologies to measure the movement of varied lipid species between or in organelle membranes, inside eukaryotic cells, including plant cells, or in bacteria, and continues in vitro or in silico approaches aiming to define, more from a biochemical and structural standpoints, how lipid transfer proteins (LTPs) or flippases/scramblases precisely function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Intracellular Lipid Transport: Methods and Protocols serves as an ideal guide for researchers seeking to shed light on diverse aspects of this critical and often elusive cellular process.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Analysis of Phosphatidylinositol Transfer at ER-PM Junctions in Receptor-Stimulated Live Cells

    Pages 1-11

    Chang, Chi-Lun (et al.)

  • Monitoring Non-vesicular Transport of Phosphatidylserine and Phosphatidylinositol 4-Phosphate in Intact Cells by BRET Analysis

    Pages 13-22

    Sohn, Mira (et al.)

  • Development of Nonspecific BRET-Based Biosensors to Monitor Plasma Membrane Inositol Lipids in Living Cells

    Pages 23-34

    Tóth, József T. (et al.)

  • Following Anterograde Transport of Phosphatidylserine in Yeast in Real Time

    Pages 35-46

    D’Ambrosio, Juan Martín (et al.)

  • Imaging Lipid Metabolism at the Golgi Complex

    Pages 47-56

    Capasso, Serena (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9136-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9135-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Intracellular Lipid Transport
Book Subtitle
Methods and Protocols
Editors
  • Guillaume Drin
Series Title
Methods in Molecular Biology
Series Volume
1949
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9136-5
DOI
10.1007/978-1-4939-9136-5
Hardcover ISBN
978-1-4939-9135-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 345
Number of Illustrations
14 b/w illustrations, 56 illustrations in colour
Topics