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Intracellular Lipid Transport

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Guillaume Drin

    1. Institut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur and CNRS, Valbonne, France

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1949)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Analysis of Phosphatidylinositol Transfer at ER-PM Junctions in Receptor-Stimulated Live Cells

    • Chi-Lun Chang, Jen Liou
    Pages 1-11

  3. Monitoring Non-vesicular Transport of Phosphatidylserine and Phosphatidylinositol 4-Phosphate in Intact Cells by BRET Analysis

    • Mira Sohn, Daniel J. Toth, Tamas Balla
    Pages 13-22

  4. Development of Nonspecific BRET-Based Biosensors to Monitor Plasma Membrane Inositol Lipids in Living Cells

    • József T. Tóth, Gergő Gulyás, László Hunyady, Péter Várnai
    Pages 23-34

  5. Following Anterograde Transport of Phosphatidylserine in Yeast in Real Time

    • Juan Martín D’Ambrosio, Véronique Albanèse, Alenka Čopič
    Pages 35-46

  6. Imaging Lipid Metabolism at the Golgi Complex

    • Serena Capasso, Giovanni D’Angelo
    Pages 47-56

  7. Advanced In Vitro Assay System to Measure Phosphatidylserine and Phosphatidylethanolamine Transport at ER/Mitochondria Interface

    • Yasushi Tamura, Rieko Kojima, Toshiya Endo
    Pages 57-67

  8. Measurement of Lipid Transport in Mitochondria by the MTL Complex

    • Juliette Jouhet, Valérie Gros, Morgane Michaud
    Pages 69-93

  9. Bi- and Trifunctional Lipids for Visualization of Sphingolipid Dynamics within the Cell

    • Doris Höglinger
    Pages 95-103

  10. Indirect Lipid Transfer Protein Activity Measurements Using Quantification of Glycosphingolipid Production

    • Anders P. E. Backman, Josefin Halin, Matti A. Kjellberg, Peter Mattjus
    Pages 105-114

  11. Measurement of Intracellular Sterol Transport in Yeast

    • Neha Chauhan, Julian A. Jentsch, Anant K. Menon
    Pages 115-136

  12. Intracellular and Plasma Membrane Cholesterol Labeling and Quantification Using Filipin and GFP-D4

    • Léa P. Wilhelm, Laetitia Voilquin, Toshihide Kobayashi, Catherine Tomasetto, Fabien Alpy
    Pages 137-152

  13. Monitoring and Modulating Intracellular Cholesterol Trafficking Using ALOD4, a Cholesterol-Binding Protein

    • Shreya Endapally, Rodney E. Infante, Arun Radhakrishnan
    Pages 153-163

  14. Measurement of Lysophospholipid Transport Across the Membrane Using Escherichia coli Spheroplasts

    • Yibin Lin, Lei Zheng, Mikhail Bogdanov
    Pages 165-180

  15. Preparation of Proteoliposomes with Purified TMEM16 Protein for Accurate Measures of Lipid Scramblase Activity

    • Janine Denise Brunner, Stephan Schenck
    Pages 181-199

  16. In Vitro Assays to Measure the Membrane Tethering and Lipid Transport Activities of the Extended Synaptotagmins

    • Xin Bian, Pietro De Camilli
    Pages 201-212

  17. Determining the Lipid-Binding Specificity of SMP Domains: An ERMES Subunit as a Case Study

    • Andrew P. AhYoung, Pascal F. Egea
    Pages 213-235

  18. In Vitro Measurement of Sphingolipid Intermembrane Transport Illustrated by GLTP Superfamily Members

    • Roopa Kenoth, Rhoderick E. Brown, Ravi Kanth Kamlekar
    Pages 237-256

  19. Purification and Characterization of Human Niemann–Pick C1 Protein

    • Xin Gong, Hongwu Qian
    Pages 257-267

  20. In Vitro Strategy to Measure Sterol/Phosphatidylinositol-4-Phosphate Exchange Between Membranes

    • Nicolas-Frédéric Lipp, Guillaume Drin
    Pages 269-292
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Keywords

About this book

This detailed book provides technical approaches to tackle a variety of questions related to intracellular lipid transport in order to improve our understanding at different scales of how lipids are accurately displaced between organelles, across long distances or at membrane contact sites, or within cellular membranes. The volume begins with methodologies to measure the movement of varied lipid species between or in organelle membranes, inside eukaryotic cells, including plant cells, or in bacteria, and continues in vitro or in silico approaches aiming to define, more from a biochemical and structural standpoints, how lipid transfer proteins (LTPs) or flippases/scramblases precisely function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Intracellular Lipid Transport: Methods and Protocols serves as an ideal guide for researchers seeking to shed light on diverse aspects of this critical and often elusive cellular process.

Editors and Affiliations

  • Institut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur and CNRS, Valbonne, France

    Guillaume Drin

Bibliographic Information

  • Book Title: Intracellular Lipid Transport

  • Book Subtitle: Methods and Protocols

  • Editors: Guillaume Drin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9136-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9135-8Published: 21 February 2019

  • eBook ISBN: 978-1-4939-9136-5Published: 21 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 345

  • Number of Illustrations: 14 b/w illustrations, 56 illustrations in colour

  • Topics: Lipidology, Membrane Biology

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