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Methods in Molecular Biology

The Extracellular Matrix

Methods and Protocols

Editors: Vigetti, Davide, Theocharis, Achilleas D. (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $149.00
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  • ISBN 978-1-4939-9133-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9132-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology.

Table of contents (21 chapters)

Table of contents (21 chapters)
  • The Complex Interplay Between Extracellular Matrix and Cells in Tissues

    Pages 1-20

    Manou, Dimitra (et al.)

  • Method for Studying ECM Expression: In Situ RT-PCR

    Pages 21-31

    CaravĂ , Elena (et al.)

  • Visualizing the Supramolecular Assembly of Collagen

    Pages 33-44

    Raspanti, Mario (et al.)

  • Steady-State and Pulse-Chase Analyses of Fibrillar Collagen

    Pages 45-53

    Forlino, Antonella (et al.)

  • Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates

    Pages 55-70

    Stelling, Mariana P. (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9133-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9132-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Extracellular Matrix
Book Subtitle
Methods and Protocols
Editors
  • Davide Vigetti
  • Achilleas D. Theocharis
Series Title
Methods in Molecular Biology
Series Volume
1952
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9133-4
DOI
10.1007/978-1-4939-9133-4
Hardcover ISBN
978-1-4939-9132-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 278
Number of Illustrations
22 b/w illustrations, 27 illustrations in colour
Topics