Methods in Molecular Biology

G Protein-Coupled Receptor Signaling

Methods and Protocols

Editors: Tiberi, Mario (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $169.00
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  • ISBN 978-1-4939-9121-1
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Hardcover $219.99
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About this book

This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening.
Chapter  4 is available open access under a CC BY 4.0 license via link.springer.com.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics

    Liao, Chenyi (et al.)

    Pages 3-19

  • Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs

    Tomobe, Katsufumi (et al.)

    Pages 21-30

  • Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies

    Bumbak, Fabian (et al.)

    Pages 31-55

  • A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties

    Zemella, Anne (et al.)

    Pages 57-77

  • Furan Cross-Linking Technology for Investigating GPCR–Ligand Interactions

    Troys, Marleen (et al.)

    Pages 81-102

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9121-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9120-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
G Protein-Coupled Receptor Signaling
Book Subtitle
Methods and Protocols
Editors
  • Mario Tiberi
Series Title
Methods in Molecular Biology
Series Volume
1947
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9121-1
DOI
10.1007/978-1-4939-9121-1
Hardcover ISBN
978-1-4939-9120-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 406
Number of Illustrations
16 b/w illustrations, 71 illustrations in colour
Topics