Methods in Molecular Biology

Collagen

Methods and Protocols

Editors: Sagi, Irit, Afratis, Nikolaos A. (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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  • ISBN 978-1-4939-9095-5
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Hardcover $199.99
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About this book

This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Cloning and Mutagenesis Strategies for Large Collagens

    Bornert, Olivier (et al.)

    Pages 3-15

  • Multi-well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen

    Krishnaswamy, Venkat Raghavan

    Pages 17-22

  • Production and Characterization of Chemically Cross-Linked Collagen Scaffolds

    Sallent, Ignacio (et al.)

    Pages 23-38

  • A Biomimetic Model for Mineralization of Type-I Collagen Fibrils

    Yao, Shasha (et al.)

    Pages 39-54

  • Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy

    Chow, Wing Ying

    Pages 57-77

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9095-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9094-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Collagen
Book Subtitle
Methods and Protocols
Editors
  • Irit Sagi
  • Nikolaos A. Afratis
Series Title
Methods in Molecular Biology
Series Volume
1944
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9095-5
DOI
10.1007/978-1-4939-9095-5
Hardcover ISBN
978-1-4939-9094-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 254
Number of Illustrations
16 b/w illustrations, 44 illustrations in colour
Topics