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Nanotechnology for Nucleic Acid Delivery

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Manfred Ogris

    1. Laboratory of MacroMolecular Cancer Therapeutics (MMCT), Department of Pharmaceutical Chemistry, Center of Pharmaceutical Sciences, Faculty of Life Sciences, University of Vienna, Vienna, Austria

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  2. Haider Sami

    1. Laboratory of MacroMolecular Cancer Therapeutics (MMCT), Department of Pharmaceutical Chemistry, Center of Pharmaceutical Sciences, Faculty of Life Sciences, University of Vienna, Vienna, Austria

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step details essential for reproducible results
  • Contains key advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1943)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection

    • Sören Reinhard, Ernst Wagner
    Pages 1-25

  3. Synthesis of Bioreducible Polycations with Controlled Topologies

    • Ye-Zi You, Jun-Jie Yan, Fei Yu, Zhi-Qiang Yu, David Oupicky
    Pages 27-38

  4. Histidine-Rich Cationic Cell-Penetrating Peptides for Plasmid DNA and siRNA Delivery

    • Antoine Kichler, A. James Mason, Arnaud Marquette, Burkhard Bechinger
    Pages 39-59

  5. Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates

    • Cornelia Lorenzer, Johannes Winkler
    Pages 61-72

  6. Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems

    • Maxim Antopolsky
    Pages 73-82

  7. Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids

    • Wolfgang Rödl, Alexander Taschauer, David Schaffert, Ernst Wagner, Manfred Ogris
    Pages 83-99

  8. Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery

    • Manon Ripoll, Patrick Neuberg, Jean-Serge Remy, Antoine Kichler
    Pages 101-122

  9. Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation

    • Michel Bessodes, Helene Dhotel, Nathalie Mignet
    Pages 123-139

  10. Preparation, Characterization, and In Vitro Evaluation of Lipidoid–Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol

    • Kaushik Thanki, Xianghui Zeng, Camilla Foged
    Pages 141-152

  11. Layer-by-Layer Assembled Nanoparticles for siRNA Delivery

    • Michaela Guter, Miriam Breunig
    Pages 153-160

  12. Layer-By-Layer Film Engineering for Sequential Gene Delivery

    • Lingxiao Xie, Yi Zou, Sean Carroll, Maria Muniz, Guangzhao Mao
    Pages 161-176

  13. Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles

    • Angela K. Pannier, Tyler Kozisek, Tatiana Segura
    Pages 177-197

  14. In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nanofilms

    • Yi Zou, Lei Wan, Jenifer Blacklock, Lingxiao Xie, Sean Carroll, David Oupicky et al.
    Pages 199-209

  15. Lyophilization of Synthetic Gene Carriers

    • Julia Christina Kasper, Sarah Hedtrich, Wolfgang Friess
    Pages 211-225

  16. Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery

    • Katharina Müller, Manfred Ogris, Haider Sami
    Pages 227-239

  17. Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles

    • Heleen Dewitte, Silke Roovers, Stefaan C. De Smedt, Ine Lentacker
    Pages 241-251

  18. Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery

    • Dialechti Vlaskou, Olga Mykhaylyk, Christian Plank
    Pages 253-290

  19. Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity

    • Ladan Parhamifar, Helene Andersen, S. Moein Moghimi
    Pages 291-299

  20. Combined Fluorimetric Caspase-3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity

    • Anna K. Larsen, Arnaldur Hall, Henrik Lundsgart, S. Moein Moghimi
    Pages 301-311
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Keywords

About this book

This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and established nanoformulations, characterization of these nanoformulations for biophysical, biological and toxicological aspects, and also protocols dealing with application and imaging of such carrier systems in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, Second Edition serves as an ideal guide to researchers seeking to use this vital area of study in their own lab work or to progress knowledge within the field itself.

Editors and Affiliations

  • Laboratory of MacroMolecular Cancer Therapeutics (MMCT), Department of Pharmaceutical Chemistry, Center of Pharmaceutical Sciences, Faculty of Life Sciences, University of Vienna, Vienna, Austria

    Manfred Ogris, Haider Sami

Bibliographic Information

  • Book Title: Nanotechnology for Nucleic Acid Delivery

  • Book Subtitle: Methods and Protocols

  • Editors: Manfred Ogris, Haider Sami

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9092-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9091-7Published: 06 March 2019

  • eBook ISBN: 978-1-4939-9092-4Published: 05 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 406

  • Number of Illustrations: 36 b/w illustrations, 71 illustrations in colour

  • Topics: Human Genetics, Nanotechnology, Biotechnology, Pharmacology/Toxicology

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