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Methods in Molecular Biology

Mouse Cell Culture

Methods and Protocols

Editors: Bertoncello, Ivan (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9086-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9085-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets.

Table of contents (21 chapters)

Table of contents (21 chapters)
  • The Applicability of Mouse Models to the Study of Human Disease

    Pages 3-22

    Rydell-Törmänen, Kristina (et al.)

  • Optimizing the Cell Culture Microenvironment

    Pages 23-30

    Bertoncello, Ivan

  • Propagation and Maintenance of Mouse Embryonic Stem Cells

    Pages 33-45

    Paynter, Jacob M. (et al.)

  • Production of High-Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells

    Pages 47-61

    Larcombe, Michael R. (et al.)

  • Generation of Mouse-Induced Pluripotent Stem Cells by Lentiviral Transduction

    Pages 63-76

    Liu, Xiaodong (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9086-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9085-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Mouse Cell Culture
Book Subtitle
Methods and Protocols
Editors
  • Ivan Bertoncello
Series Title
Methods in Molecular Biology
Series Volume
1940
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9086-3
DOI
10.1007/978-1-4939-9086-3
Hardcover ISBN
978-1-4939-9085-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 315
Number of Illustrations
9 b/w illustrations, 55 illustrations in colour
Topics