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Methods in Molecular Biology

Viral Vectors for Gene Therapy

Methods and Protocols

Editors: Manfredsson, Fredric P., Benskey, Matthew J. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9065-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9064-1
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  • Usually dispatched within 3 to 5 business days.
About this book

This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, construction, and application of transcription activation-like effectors; multi-modal production of adeno-associated virus; construction of oncolytic herpes simplex virus; AAV-mediated gene delivery to the mouse liver; and intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Viral Vectors for Gene Therapy: Methods and Protocols is a valuable resource for researchers, clinicians, and students looking to utilize viral vectors in gene therapy experiments.

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Basic Concepts in Viral Vector-Mediated Gene Therapy

    Pages 3-26

    Benskey, Matthew J. (et al.)

  • Design and Assembly of CRISPR/Cas9 Lentiviral and rAAV Vectors for Targeted Genome Editing

    Pages 29-45

    Sandoval, Ivette M. (et al.)

  • Design, Construction, and Application of Transcription Activation-Like Effectors

    Pages 47-58

    Deng, Peter (et al.)

  • Practical Considerations for the Use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain

    Pages 59-87

    Aldrin-Kirk, Patrick (et al.)

  • AAV Production Using Baculovirus Expression Vector System

    Pages 91-99

    Sandro, Quentin (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9065-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9064-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Viral Vectors for Gene Therapy
Book Subtitle
Methods and Protocols
Editors
  • Fredric P. Manfredsson
  • Matthew J. Benskey
Series Title
Methods in Molecular Biology
Series Volume
1937
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9065-8
DOI
10.1007/978-1-4939-9065-8
Hardcover ISBN
978-1-4939-9064-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 328
Number of Illustrations
13 b/w illustrations, 33 illustrations in colour
Topics