Post-Translational Modification of Proteins

1. Front Matter

   Pages i-xi

   PDF

2. Web-Based Computational Tools for the Prediction and Analysis of Posttranslational Modifications of Proteins

   • Vladimir A. Ivanisenko, Timofey V. Ivanisenko, Olga V. Saik, Pavel S. Demenkov, Dmitry A. Afonnikov, Nikolay A. Kolchanov

   Pages 1-20

3. Detection of Posttranslational Modifications by Fluorescent Staining of Two-Dimensional Gels

   • Archana M. Jastorff, Chris W. Turck

   Pages 21-32

4. Analysis of Ser/Thr-Linked Sugar Chains

   • Kalyan R. Anumula

   Pages 33-42

5. Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling

   • Detlef Grunow, Véronique Blanchard

   Pages 43-49

6. Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry: Detection of Lewis X Structures

   • Christoph Kannicht, Detlef Grunow, Lothar Lucka

   Pages 51-64

7. Chromatographic Profiling of N-Glycans

   • Martin Gohlke, Véronique Blanchard

   Pages 65-81

8. Activity Determination of Glycosyltransferases

   • Kalyan R. Anumula

   Pages 83-92

9. Characterization of Site-Specific N-Glycosylation

   • Helga Hevér, Zsuzsanna Darula, Katalin F. Medzihradszky

   Pages 93-125

10. Glycosylation of Type I Collagen

    • Mitsuo Yamauchi, Marnisa Sricholpech, Masahiko Terajima, Kenneth B. Tomer, Irina Perdivara

    Pages 127-144

11. Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa

    • Nahid Azzouz, Peter Gerold, Ralph T. Schwarz

    Pages 145-162

12. Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods

    • Christof Lenz

    Pages 163-178

13. Mass Spectrometry-Based Method for Detection and Identification of Free Thiol Groups in Proteins

    • Barbara A. Solecka-Witulska, Christoph Weise, Christoph Kannicht

    Pages 179-189

14. Mass Spectrometric Determination of Protein Ubiquitination

    • Carol E. Parker, Maria R. E. Warren Hines, Viorel Mocanu, Susanna F. Greer, Christoph H. Borchers

    Pages 191-221

15. Analysis of Sumoylation

    • Jan Breucker, Andrea Pichler

    Pages 223-233

16. An Approach for the Identification of Proteins Modified with ISG15

    • Tomoharu Takeuchi, Shun Koinuma, Hideyoshi Yokosawa, Yoichiro Arata

    Pages 235-246

17. α-Amidated Peptides: Approaches for Analysis

    • Gregory P. Mueller, Rachel C. Lazarus, William J. Driscoll

    Pages 247-264

18. S-Acylation of Proteins

    • Larisa Kordyukova, Ludwig Krabben, Marina Serebryakova, Michael Veit

    Pages 265-291

19. Analysis of Methylation, Acetylation, and Other Modifications in Bacterial Ribosomal Proteins

    • Randy J. Arnold, Suraj Saraswat, James P. Reilly

    Pages 293-307

20. Lysine Hydroxylation and Cross-Linking of Collagen

    • Mitsuo Yamauchi, Masahiko Terajima, Masashi Shiiba

    Pages 309-324

The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins.  In Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting edge, Post-Translational Modifications of Proteins: Tools for Functional Proteomics,Third Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.

• protein function
• structure-function relationship
• protein-ligand binding
• phosphoproteomics
• cell culture
• protein ubiquitination

• Recombinant Research and Development, Octapharma Biopharmaceuticals GmbH, Heidelberg, Germany

  Christoph Kannicht

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