Post-Translational Modification of Proteins
Tools for Functional Proteomics
Editors: Kannicht, Christoph (Ed.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting edge, Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.
- Table of contents (19 chapters)
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Web-Based Computational Tools for the Prediction and Analysis of Posttranslational Modifications of Proteins
Pages 1-20
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Detection of Posttranslational Modifications by Fluorescent Staining of Two-Dimensional Gels
Pages 21-32
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Analysis of Ser/Thr-Linked Sugar Chains
Pages 33-42
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Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling
Pages 43-49
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Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry: Detection of Lewis X Structures
Pages 51-64
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Table of contents (19 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Post-Translational Modification of Proteins
- Book Subtitle
- Tools for Functional Proteomics
- Editors
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- Christoph Kannicht
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1934
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9055-9
- DOI
- 10.1007/978-1-4939-9055-9
- Hardcover ISBN
- 978-1-4939-9053-5
- Series ISSN
- 1064-3745
- Edition Number
- 3
- Number of Pages
- XI, 327
- Number of Illustrations
- 81 b/w illustrations, 31 illustrations in colour
- Topics
