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Legionella

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Carmen Buchrieser

    1. Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

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    You can also search for this editor in PubMed   Google Scholar

  2. Hubert Hilbi

    1. Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1921)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xxvi
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  2. Ecology and Physiology of Legionella

    1. Front Matter

      Pages 1-1
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    2. Analysis of Iron Requirements and Siderophore Production

      • Denise M. Burnside, Nicholas P. Cianciotto
      Pages 3-19

    3. The Pathometabolism of Legionella Studied by Isotopologue Profiling

      • Klaus Heuner, Mareike Kunze, Fan Chen, Wolfgang Eisenreich
      Pages 21-44

    4. Quantification of Viable but Non-Culturable Cells of Legionella pneumophila

      • Rosalie S. Braun, Nilmini Mendis, Laam Li, Sebastien P. Faucher
      Pages 45-53

    5. Selection of Legionella Virulence-Related Traits by Environmental Protozoa

      • Francisco Amaro, Howard Shuman
      Pages 55-78

    6. Migration of Acanthamoeba castellanii Through Legionella Biofilms

      • Ramon Hochstrasser, Hubert Hilbi
      Pages 79-89

  3. Legionella Genetics

    1. Front Matter

      Pages 91-91
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    2. Scar-Free Genome Editing in Legionella pneumophila

      • Nathalie Bailo, Hussein Kanaan, Elisabeth Kay, Xavier Charpentier, Patricia Doublet, Christophe Gilbert
      Pages 93-105

    3. Querying Legionella Genomes Using Transposition-Sequencing

      • Léo Hardy, Xavier Charpentier
      Pages 107-122

    4. Screening Targeted Legionella pneumophila Mutant Libraries In Vivo Using INSeq

      • Stephanie R. Shames
      Pages 123-144

    5. Genetic Manipulation of Non-pneumophila Legionella: Protocols Developed for Legionella longbeachae

      • Elizabeth Korevaar, Chen Ai Khoo, Hayley J. Newton
      Pages 145-157

  4. Cellular Microbiology of Legionella

    1. Front Matter

      Pages 159-159
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    2. Quantitative Imaging Flow Cytometry of Legionella-Containing Vacuoles in Dually Fluorescence-Labeled Dictyostelium

      • Amanda Welin, Stephen Weber, Hubert Hilbi
      Pages 161-177

    3. Sorting of Phagocytic Cells Infected with Legionella pneumophila

      • Monica Rolando, Carmen Buchrieser
      Pages 179-189

    4. Single Cell Analysis of Legionella and Legionella-Infected Acanthamoeba by Agarose Embedment

      • Nicolas Personnic, Bianca Striednig, Hubert Hilbi
      Pages 191-204

    5. Mitochondrial Dynamics and Activity in Legionella-Infected Cells

      • Ok-Ryul Song, Priscille Brodin, Carmen Buchrieser, Pedro Escoll
      Pages 205-220

    6. Perturbation of Legionella Cell Infection by RNA Interference

      • Bernhard Steiner, A. Leoni Swart, Hubert Hilbi
      Pages 221-238

  5. Biochemical Assays to Study Legionella Effectors and Enzymes

    1. Front Matter

      Pages 239-239
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    2. Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila

      • Tomoko Kubori, Hiroki Nagai
      Pages 241-247
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Keywords

About this book

The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila.  Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella,  and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.

Editors and Affiliations

  • Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

    Carmen Buchrieser

  • Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland

    Hubert Hilbi

Bibliographic Information

  • Book Title: Legionella

  • Book Subtitle: Methods and Protocols

  • Editors: Carmen Buchrieser, Hubert Hilbi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9048-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9047-4Published: 29 January 2019

  • eBook ISBN: 978-1-4939-9048-1Published: 29 January 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XXVI, 476

  • Number of Illustrations: 24 b/w illustrations, 50 illustrations in colour

  • Topics: Infectious Diseases, Immunology

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