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Methods in Molecular Biology

T-Cell Motility

Methods and Protocols

Editors: Verma, Navin Kumar (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9036-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-9035-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields of immunology, T-cell biology, biochemistry, molecular biology, and imaging.


Table of contents (18 chapters)

Table of contents (18 chapters)
  • An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility

    Verma, Navin Kumar (et al.)

    Pages 1-9

  • Isolation of Human Peripheral Blood T-Lymphocytes

    Kizhakeyil, Atish (et al.)

    Pages 11-17

  • A Laboratory Model to Study T-Cell Motility

    Verma, Navin Kumar (et al.)

    Pages 19-23

  • Quantification of T-Cell Migratory Phenotypes Using High-Content Analysis

    Ng, Aik Seng (et al.)

    Pages 25-32

  • Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time

    Ong, Seow Theng (et al.)

    Pages 33-40

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9036-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-9035-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
T-Cell Motility
Book Subtitle
Methods and Protocols
Editors
  • Navin Kumar Verma
Series Title
Methods in Molecular Biology
Series Volume
1930
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9036-8
DOI
10.1007/978-1-4939-9036-8
Hardcover ISBN
978-1-4939-9035-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 158
Number of Illustrations
3 b/w illustrations, 28 illustrations in colour
Topics