- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields of immunology, T-cell biology, biochemistry, molecular biology, and imaging.
- Table of contents (18 chapters)
-
-
An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility
Pages 1-9
-
Isolation of Human Peripheral Blood T-Lymphocytes
Pages 11-17
-
A Laboratory Model to Study T-Cell Motility
Pages 19-23
-
Quantification of T-Cell Migratory Phenotypes Using High-Content Analysis
Pages 25-32
-
Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time
Pages 33-40
-
Table of contents (18 chapters)
Buy this book
Services for this Book
Recommended for you
Bibliographic Information
- Bibliographic Information
-
- Book Title
- T-Cell Motility
- Book Subtitle
- Methods and Protocols
- Editors
-
- Navin Kumar Verma
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1930
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9036-8
- DOI
- 10.1007/978-1-4939-9036-8
- Hardcover ISBN
- 978-1-4939-9035-1
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 158
- Number of Illustrations
- 3 b/w illustrations, 28 illustrations in colour
- Topics
