Methods in Molecular Biology

Cancer Metabolism

Methods and Protocols

Editors: Haznadar, Majda (Ed.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essentail for reproducible results 
  • Contains key note and implementation advice from the experts
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eBook $219.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9027-6
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Hardcover $279.99
price for USA in USD
  • ISBN 978-1-4939-9026-9
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  • Usually dispatched within 3 to 5 business days.
About this book

This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism.  Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism.  The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cancer Metabolism: Methods and Protocols aims to be an essential compilation of protocols utilized in many laboratories.

Table of contents (25 chapters)

Table of contents (25 chapters)
  • Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction

    Crooks, Daniel R. (et al.)

    Pages 1-27

  • Imaging Cancer Metabolism with Positron Emission Tomography (PET)

    Witney, Timothy H. (et al.)

    Pages 29-44

  • Radioluminescence Microscopy: A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells

    Sengupta, Debanti (et al.)

    Pages 45-53

  • Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation Dynamics in Cancer Cell Metabolism and Epigenetics

    Newman, Alice C. (et al.)

    Pages 55-67

  • Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM)

    Creed, Sarah (et al.)

    Pages 69-76

Buy this book

eBook $219.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9027-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
  • ISBN 978-1-4939-9026-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cancer Metabolism
Book Subtitle
Methods and Protocols
Editors
  • Majda Haznadar
Series Title
Methods in Molecular Biology
Series Volume
1928
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9027-6
DOI
10.1007/978-1-4939-9027-6
Hardcover ISBN
978-1-4939-9026-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 495
Number of Illustrations
19 b/w illustrations, 60 illustrations in colour
Topics