Methods in Molecular Biology

Recombinant Protein Production in Yeast

Editors: Gasser, Brigitte, Mattanovich, Diethard (Eds.)

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  • Include cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-9024-5
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Hardcover $159.99
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About this book

This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications. 

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Table of contents (20 chapters)

Table of contents (20 chapters)
  • Established and Upcoming Yeast Expression Systems

    Gündüz Ergün, Burcu (et al.)

    Pages 1-74

  • Detection and Elimination of Cellular Bottlenecks in Protein-Producing Yeasts

    Zahrl, Richard J. (et al.)

    Pages 75-95

  • Pichia pastoris Promoters

    Türkanoğlu Özçelik, Aysun (et al.)

    Pages 97-112

  • Selection of the Optimal Yeast Host for the Synthesis of Recombinant Enzymes

    Bischoff, Felix (et al.)

    Pages 113-132

  • Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha

    Yoo, Su Jin (et al.)

    Pages 133-151

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9024-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-4939-9023-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Recombinant Protein Production in Yeast
Editors
  • Brigitte Gasser
  • Diethard Mattanovich
Series Title
Methods in Molecular Biology
Series Volume
1923
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9024-5
DOI
10.1007/978-1-4939-9024-5
Hardcover ISBN
978-1-4939-9023-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 431
Number of Illustrations
14 b/w illustrations, 46 illustrations in colour
Topics