- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume provides methods and approaches to study genetic and environmental regulatory controls on odontogenesis. Chapters guide readers through protocols for isolation and characterization of both epithelial and mesenchymal dental cells, methods on isolation, phenotypic characterization, expansion, differentiation, immunofluorescence, in situ hybridization, immunohistochemistry, imaging protocols, rodent dental fluorosis model, 3D assessment of crown size, dental diseases models, next generation sequencing, genetic and epigenetic studies, genome-wide association studies as well as clinical protocols for measurement of early childhood caries and saliva, and supragingival fluids and biofilm collection and subsequent analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Odontogenesis: Methods and Protocols aims to guide researchers towards elucidating the secrets and mysteries of a fascinating and unique organ, the tooth.
- Table of contents (41 chapters)
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Microdissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors
Pages 3-11
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Establishment of an Immortalized Mouse Bmp2 Knockout Dental Papilla Mesenchymal Cell Line
Pages 13-19
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Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells
Pages 21-27
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Isolation of Dental Stem Cell-Enriched Populations from Continuously Growing Mouse Incisors
Pages 29-37
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Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentinogenesis
Pages 39-48
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Table of contents (41 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Odontogenesis
- Book Subtitle
- Methods and Protocols
- Editors
-
- Petros Papagerakis
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1922
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9012-2
- DOI
- 10.1007/978-1-4939-9012-2
- Hardcover ISBN
- 978-1-4939-9011-5
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XVIII, 565
- Number of Illustrations
- 17 b/w illustrations, 135 illustrations in colour
- Topics
