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Methods in Molecular Biology

Odontogenesis

Methods and Protocols

Editors: Papagerakis, Petros (Ed.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $219.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9012-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
  • ISBN 978-1-4939-9011-5
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume provides methods and approaches to study genetic and environmental regulatory controls on odontogenesis. Chapters guide readers through protocols for isolation and characterization of both epithelial and mesenchymal dental cells, methods on isolation, phenotypic characterization, expansion, differentiation, immunofluorescence, in situ hybridization, immunohistochemistry, imaging protocols, rodent dental fluorosis model, 3D assessment of crown size, dental diseases models, next generation sequencing, genetic and epigenetic studies, genome-wide association studies as well as clinical protocols for measurement of early childhood caries and saliva,  and supragingival fluids and biofilm collection and subsequent analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Odontogenesis: Methods and Protocols aims to guide researchers towards elucidating the secrets and mysteries of a fascinating and unique organ, the tooth.

Table of contents (41 chapters)

Table of contents (41 chapters)
  • Microdissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors

    Pages 3-11

    Harada, Hidemitsu (et al.)

  • Establishment of an Immortalized Mouse Bmp2 Knockout Dental Papilla Mesenchymal Cell Line

    Pages 13-19

    Xu, Wen’an (et al.)

  • Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells

    Pages 21-27

    Guirado, Elizabeth (et al.)

  • Isolation of Dental Stem Cell-Enriched Populations from Continuously Growing Mouse Incisors

    Pages 29-37

    Balic, Anamaria

  • Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentinogenesis

    Pages 39-48

    Jing, Yan (et al.)

Buy this book

eBook $219.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9012-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
  • ISBN 978-1-4939-9011-5
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Odontogenesis
Book Subtitle
Methods and Protocols
Editors
  • Petros Papagerakis
Series Title
Methods in Molecular Biology
Series Volume
1922
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9012-2
DOI
10.1007/978-1-4939-9012-2
Hardcover ISBN
978-1-4939-9011-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVIII, 565
Number of Illustrations
17 b/w illustrations, 135 illustrations in colour
Topics