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Methods in Molecular Biology

Vertebrate Embryogenesis

Embryological, Cellular, and Genetic Methods

Editors: Pelegri, Francisco (Ed.)

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  • Includes cutting-edge, fully updated techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $169.00
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  • ISBN 978-1-4939-9009-2
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Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9008-5
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About this book

This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development.  
Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Table of contents (26 chapters)

Table of contents (26 chapters)
  • Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments

    Pages 1-16

    Houston, Douglas W.

  • Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos

    Pages 17-32

    Nguyen, Thao (et al.)

  • Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos

    Pages 33-39

    Sheets, Michael D.

  • Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts

    Pages 41-57

    Tokmakov, Alexander A. (et al.)

  • Membrane Microdomains as Platform to Study Membrane-Associated Events During Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis

    Pages 59-73

    Sato, Ken-Ichi (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9009-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9008-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Vertebrate Embryogenesis
Book Subtitle
Embryological, Cellular, and Genetic Methods
Editors
  • Francisco Pelegri
Series Title
Methods in Molecular Biology
Series Volume
1920
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9009-2
DOI
10.1007/978-1-4939-9009-2
Hardcover ISBN
978-1-4939-9008-5
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 434
Number of Illustrations
12 b/w illustrations, 70 illustrations in colour
Topics