- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
The third edition of this book provides a large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research.
- Table of contents (35 chapters)
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Isolation and Generation of Osteoclasts
Pages 3-19
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Isolation and Generation of Osteoblasts
Pages 21-38
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Isolation, Purification, Generation, and Culture of Osteocytes
Pages 39-51
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Isolation, Differentiation, and Characterization of Human Bone Marrow Stem Cells In Vitro and In Vivo
Pages 53-70
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2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies
Pages 71-98
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Table of contents (35 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Bone Research Protocols
- Editors
-
- Aymen I. Idris
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1914
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8997-3
- DOI
- 10.1007/978-1-4939-8997-3
- Hardcover ISBN
- 978-1-4939-8996-6
- Series ISSN
- 1064-3745
- Edition Number
- 3
- Number of Pages
- XV, 670
- Number of Illustrations
- 51 b/w illustrations, 121 illustrations in colour
- Topics