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Plant Genome Editing with CRISPR Systems

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Yiping Qi

    1. Department of Plant Science and Landscape Architecture, University of Maryland College Park, College Park, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1917)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xv
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  2. Review on Plant DNA Repair and Genome Editing

    1. Front Matter

      Pages 1-1
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    2. Plant DNA Repair Pathways and Their Applications in Genome Engineering

      • Qiudeng Que, Zhongying Chen, Tim Kelliher, David Skibbe, Shujie Dong, Mary-Dell Chilton
      Pages 3-24

  3. CRISPR Design and Mutation Analysis

    1. Front Matter

      Pages 25-25
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    2. Rapid Screening of CRISPR/Cas9-Induced Mutants Using the ACT-PCR Method

      • Chun Wang, Kejian Wang
      Pages 27-32

    3. Decoding Sanger Sequencing Chromatograms from CRISPR-Induced Mutations

      • Xianrong Xie, Xingliang Ma, Yao-Guang Liu
      Pages 33-43

  4. Multiplexed CRISPR-Cas9 Systems

    1. Front Matter

      Pages 45-45
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    2. Creating Large Chromosomal Deletions in Rice Using CRISPR/Cas9

      • Riqing Li, Si Nian Char, Bing Yang
      Pages 47-61

    3. A Multiplexed CRISPR/Cas9 Editing System Based on the Endogenous tRNA Processing

      • Kabin Xie, Yinong Yang
      Pages 63-73

    4. A Single Transcript CRISPR-Cas9 System for Multiplex Genome Editing in Plants

      • Xu Tang, Zhaohui Zhong, Qiurong Ren, Binglin Liu, Yong Zhang
      Pages 75-82

    5. CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation

      • Aimee Malzahn, Yong Zhang, Yiping Qi
      Pages 83-93

  5. CRISPR-Cas9 Editing in Monocots

    1. Front Matter

      Pages 95-95
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    2. Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR/Cas9

      • Weihang Gu, Dabing Zhang, Yiping Qi, Zheng Yuan
      Pages 97-107

    3. Knocking Out MicroRNA Genes in Rice with CRISPR-Cas9

      • Jianping Zhou, Zhaohui Zhong, Hongqiao Chen, Qian Li, Xuelian Zheng, Yiping Qi et al.
      Pages 109-119

    4. An Agrobacterium-Mediated CRISPR/Cas9 Platform for Genome Editing in Maize

      • Keunsub Lee, Huilan Zhu, Bing Yang, Kan Wang
      Pages 121-143

  6. CRISPR-Cas9 Editing in Dicots

    1. Front Matter

      Pages 145-145
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    2. Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited Arabidopsis Plants

      • Hanchuanzhi Yu, Yunde Zhao
      Pages 147-154

    3. Creating Targeted Gene Knockouts in Brassica oleracea Using CRISPR/Cas9

      • Tom Lawrenson, Penny Hundleby, Wendy Harwood
      Pages 155-170

    4. Application of CRISPR/Cas9-Mediated Gene Editing in Tomato

      • Nathan T. Reem, Joyce Van Eck
      Pages 171-182

    5. Genome Editing in Potato with CRISPR/Cas9

      • Satya Swathi Nadakuduti, Colby G. Starker, Daniel F. Voytas, C. Robin Buell, David S. Douches
      Pages 183-201
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Keywords

About this book

This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

Editors and Affiliations

  • Department of Plant Science and Landscape Architecture, University of Maryland College Park, College Park, USA

    Yiping Qi

Bibliographic Information

  • Book Title: Plant Genome Editing with CRISPR Systems

  • Book Subtitle: Methods and Protocols

  • Editors: Yiping Qi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8991-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8990-4Published: 05 January 2019

  • eBook ISBN: 978-1-4939-8991-1Published: 04 January 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 367

  • Number of Illustrations: 8 b/w illustrations, 65 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

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