Plant Genome Editing with CRISPR Systems
Methods and Protocols
Editors: Qi, Yiping (Ed.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
- Table of contents (26 chapters)
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Plant DNA Repair Pathways and Their Applications in Genome Engineering
Pages 3-24
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Rapid Screening of CRISPR/Cas9-Induced Mutants Using the ACT-PCR Method
Pages 27-32
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Decoding Sanger Sequencing Chromatograms from CRISPR-Induced Mutations
Pages 33-43
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Creating Large Chromosomal Deletions in Rice Using CRISPR/Cas9
Pages 47-61
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A Multiplexed CRISPR/Cas9 Editing System Based on the Endogenous tRNA Processing
Pages 63-73
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Plant Genome Editing with CRISPR Systems
- Book Subtitle
- Methods and Protocols
- Editors
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- Yiping Qi
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1917
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8991-1
- DOI
- 10.1007/978-1-4939-8991-1
- Hardcover ISBN
- 978-1-4939-8990-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 367
- Number of Illustrations
- 8 b/w illustrations, 65 illustrations in colour
- Topics
