Methods in Molecular Biology

Plant Genome Editing with CRISPR Systems

Methods and Protocols

Editors: Qi, Yiping (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
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  • ISBN 978-1-4939-8991-1
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Hardcover $219.99
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  • ISBN 978-1-4939-8990-4
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About this book

This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

Table of contents (26 chapters)

  • Plant DNA Repair Pathways and Their Applications in Genome Engineering

    Que, Qiudeng (et al.)

    Pages 3-24

  • Rapid Screening of CRISPR/Cas9-Induced Mutants Using the ACT-PCR Method

    Wang, Chun (et al.)

    Pages 27-32

  • Decoding Sanger Sequencing Chromatograms from CRISPR-Induced Mutations

    Xie, Xianrong (et al.)

    Pages 33-43

  • Creating Large Chromosomal Deletions in Rice Using CRISPR/Cas9

    Li, Riqing (et al.)

    Pages 47-61

  • A Multiplexed CRISPR/Cas9 Editing System Based on the Endogenous tRNA Processing

    Xie, Kabin (et al.)

    Pages 63-73

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8991-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8990-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Plant Genome Editing with CRISPR Systems
Book Subtitle
Methods and Protocols
Editors
  • Yiping Qi
Series Title
Methods in Molecular Biology
Series Volume
1917
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8991-1
DOI
10.1007/978-1-4939-8991-1
Hardcover ISBN
978-1-4939-8990-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 367
Number of Illustrations
8 b/w illustrations, 65 illustrations in colour
Topics