- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
-
This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to calpain substrate identification. The chapters in this book are divided into five parts and cover topics such as production and purification of calpains; determination of calpain localization, expression, and activity; identification of calpain-activated protein function; interrogation of calpastatin; and manipulation of calpain expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Calpain: Methods and Protocols is a valuable resource for researchers and scientists who want to learn more about this developing field, and get inspired to make new discoveries that will aid in diagnosing and treating calpain-related diseases.
- Table of contents (20 chapters)
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Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin Using Anion-Exchange Chromatography
Pages 3-11
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Bacterial Expression and Purification of Calpains
Pages 13-27
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Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity
Pages 31-38
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FRET-Based Assays to Determine Calpain Activity
Pages 39-55
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In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer
Pages 57-66
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Table of contents (20 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Calpain
- Book Subtitle
- Methods and Protocols
- Editors
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- Jeannette S. Messer
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1915
- Copyright
- 2019
- Publisher
- Springer-Verlag New York
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8988-1
- DOI
- 10.1007/978-1-4939-8988-1
- Hardcover ISBN
- 978-1-4939-8987-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 276
- Number of Illustrations
- 29 b/w illustrations, 37 illustrations in colour
- Topics
