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Co-Immunoprecipitation Methods for Brain Tissue

  • Book
  • © 2019

Overview

Editors:
  1. Yuji Odagaki

    1. Department of Psychiatry, Saitama Medical University, Saitama, Japan

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    You can also search for this editor in PubMed   Google Scholar

  2. Dasiel O. Borroto-Escuela

    1. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 144)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Co-immunoprecipitation Methods for Detection of G Protein-Coupled Receptors in Brain Tissue

    • Kazunori Namba, Hiroki Kaneko
    Pages 1-8

  3. Co-immunoprecipitation Methods to Identify Associated Proteins with Estrogen Receptor α at Postsynaptic Density in Brain Tissue

    • Gen Murakami, Suguru Kawato
    Pages 9-21

  4. The Use of Co-immunoprecipitation to Study Conformation-Specific Protein Interactions of Oligomeric α-Synuclein Aggregates

    • Cristine Betzer, Rikke Hahn Kofoed, Poul Henning Jensen
    Pages 23-36

  5. Using Co-immunoprecipitation and Shotgun Mass Spectrometry for Protein-Protein Interaction Identification in Cultured Human Oligodendrocytes

    • Bradley Smith, Daniel Martins-de-Souza, Mariana Fioramonte
    Pages 37-47

  6. Co-immunoprecipitation Analysis of GPCR Complexes in the Central Nervous System

    • Yuji Kamikubo, Takashi Sakurai
    Pages 49-64

  7. The Histoblot Technique: A Reliable Approach to Analyze Expression Profile of Proteins and to Predict Their Molecular Association

    • Carolina Aguado, Rafael Luján
    Pages 65-88

  8. Co-immunoprecipitation Assay to Investigate the Interaction Strength Between Synaptic Proteins Using COS-7 Cells

    • Sosuke Yagishita
    Pages 89-96

  9. Guanosine-5′-O-(3-[35S]thio)triphosphate ([35S]GTPγS) Binding/Immunoprecipitation Assay Using Magnetic Beads Coated with Anti-Gα Antibody in Mammalian Brain Membranes

    • Yuji Odagaki
    Pages 97-107

  10. Co-immunoprecipitation as a Useful Tool for Detection of G Protein-Coupled Receptor Oligomers

    • Kirill Shumilov, Alejandra Valderrama-Carvajal, María García-Bonilla, Alicia Rivera
    Pages 109-122

  11. Isolation and Detection of G Protein-Coupled Receptor (GPCR) Heteroreceptor Complexes in Rat Brain Synaptosomal Preparation Using a Combined Brain Subcellular Fractionation/Co-immunoprecipitation (Co-IP) Procedures

    • Dasiel O. Borroto-Escuela, Manuel Narvaez, Martina Zannoni, Chiara Contri, Minerva Crespo-Ramírez, Michael di Palma et al.
    Pages 123-135

  12. Co-immunoprecipitation of Membrane-Bound Receptors from Subsynaptic Compartments

    • Wilber Romero-Fernandez, Maria Garcia-Barcelo, Yunis Perez-Betancourt
    Pages 137-145

  13. Coimmunoprecipitation (co-IP) Analysis for Protein-Protein Interactions in the Neurons of the Cerebral Ganglia of the Land Snails of the Genus Polymita During Aestivation

    • Daily Y. Borroto-Escuela, Idania Hernández-Ramos, Kjell Fuxe, Dasiel O. Borroto-Escuela
    Pages 147-156

  14. Co-immunoprecipitation (Co-IP) of G Protein-Coupled Receptor (GPCR)-Receptor Tyrosine Kinase (RTK) Complexes from the Dorsal Hippocampus of the Rat Brain

    • Michael Di Palma, Manuel Narvaez, Mariana Pita-Rodríguez, Chiara Contri, Martina Zannoni, Riccardo Cuppini et al.
    Pages 157-164

  15. Back Matter

    Pages 165-166
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Keywords

About this book

This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System. The chapters in this book discuss topics such as using co-IP to detect G protein-coupled receptors (GPCR), receptor tyrosine kinases (RTK) and ion channels heteroreceptor complexes in brain tissue; the histoblot technique; interaction strength between synaptic proteins using COS-7; and co-IP analysis of the protein-protein interactions in the neurons of Polymita. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and thorough, Co-Immunoprecipitation Methods for Brain Tissue is a valuable resource for any researcher interesting in learning more about this developing field.



Editors and Affiliations

  • Department of Psychiatry, Saitama Medical University, Saitama, Japan

    Yuji Odagaki

  • Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    Dasiel O. Borroto-Escuela

Bibliographic Information

  • Book Title: Co-Immunoprecipitation Methods for Brain Tissue

  • Editors: Yuji Odagaki, Dasiel O. Borroto-Escuela

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-8985-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8984-3Published: 14 December 2018

  • eBook ISBN: 978-1-4939-8985-0Published: 13 December 2018

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIII, 166

  • Number of Illustrations: 9 b/w illustrations, 19 illustrations in colour

  • Topics: Neurosciences, Protein Science

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