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Neuromethods

Co-Immunoprecipitation Methods for Brain Tissue

Editors: Odagaki, Yuji, Borroto-Escuela, Dasiel (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System. The chapters in this book discuss topics such as using co-IP to detect G protein-coupled receptors (GPCR), receptor tyrosine kinases (RTK) and ion channels heteroreceptor complexes in brain tissue; the histoblot technique; interaction strength between synaptic proteins using COS-7; and co-IP analysis of the protein-protein interactions in the neurons of Polymita. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and thorough, Co-Immunoprecipitation Methods for Brain Tissue is a valuable resource for any researcher interesting in learning more about this developing field.


Table of contents (13 chapters)

Table of contents (13 chapters)
  • Co-immunoprecipitation Methods for Detection of G Protein-Coupled Receptors in Brain Tissue

    Pages 1-8

    Namba, Kazunori (et al.)

  • Co-immunoprecipitation Methods to Identify Associated Proteins with Estrogen Receptor α at Postsynaptic Density in Brain Tissue

    Pages 9-21

    Murakami, Gen (et al.)

  • The Use of Co-immunoprecipitation to Study Conformation-Specific Protein Interactions of Oligomeric α-Synuclein Aggregates

    Pages 23-36

    Betzer, Cristine (et al.)

  • Using Co-immunoprecipitation and Shotgun Mass Spectrometry for Protein-Protein Interaction Identification in Cultured Human Oligodendrocytes

    Pages 37-47

    Smith, Bradley (et al.)

  • Co-immunoprecipitation Analysis of GPCR Complexes in the Central Nervous System

    Pages 49-64

    Kamikubo, Yuji (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8985-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-8984-3
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Co-Immunoprecipitation Methods for Brain Tissue
Editors
  • Yuji Odagaki
  • Dasiel Borroto-Escuela
Series Title
Neuromethods
Series Volume
144
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8985-0
DOI
10.1007/978-1-4939-8985-0
Hardcover ISBN
978-1-4939-8984-3
Series ISSN
0893-2336
Edition Number
1
Number of Pages
XIII, 166
Number of Illustrations
9 b/w illustrations, 19 illustrations in colour
Topics

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