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Methods in Molecular Biology

Immune Checkpoint Blockade

Methods and Protocols

Editors: Pico de Coaña, Yago (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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About this book

This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor microenvironment and evaluating the immune system at a systemic level, the book continues by covering functional assays that provide answers to questions that may be raised after studying the immune system and its responses to immunotherapies, as well as the use of animal models in this research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Immune Checkpoint Blockade: Methods and Protocols serves to aid researchers in furthering our understanding of checkpoint blockage as well as the study of tumor immunology and the development of new immunotherapies.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Double Immunohistochemistry and Digital Image Analysis

    Pages 3-11

    Moreno-Ruiz, Pablo (et al.)

  • Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Subclassification of Tumor-Infiltrating and Surrounding Immune Cells

    Pages 13-31

    Wickenhauser, Claudia (et al.)

  • Systems-Level Immune Monitoring by Mass Cytometry

    Pages 33-48

    Lakshmikanth, Tadepally (et al.)

  • Immune Monitoring of Cancer Patients by Multi-color Flow Cytometry

    Pages 49-65

    Neo, Shi Yong (et al.)

  • High-Throughput, Fast, and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry

    Pages 67-79

    Marino, Fabio (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-4939-8979-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-8978-2
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Immune Checkpoint Blockade
Book Subtitle
Methods and Protocols
Editors
  • Yago Pico de Coaña
Series Title
Methods in Molecular Biology
Series Volume
1913
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8979-9
DOI
10.1007/978-1-4939-8979-9
Hardcover ISBN
978-1-4939-8978-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 225
Number of Illustrations
15 b/w illustrations, 35 illustrations in colour
Topics

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