Methods in Molecular Biology

Hepatic Stem Cells

Methods and Protocols

Editors: Tanimizu, Naoki (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
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  • ISBN 978-1-4939-8961-4
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Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-8960-7
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About this book

This volume looks at the liver’s epithelial cells—hepatocytes and cholangiocytes—and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology.


Table of contents (21 chapters)

  • Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells

    Tsuchiya, Atsunori (et al.)

    Pages 3-8

  • Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse Liver

    Tanimizu, Naoki

    Pages 9-17

  • Identification and Isolation of Clonogenic Cholangiocyte in Mouse

    Li, Bin (et al.)

    Pages 19-27

  • Isolation and Expansion of Rat Hepatocytic Progenitor Cells

    Kino, Junichi (et al.)

    Pages 29-41

  • Genetic Lineage Tracing of Biliary Epithelial Cells

    Rubio-Tomás, Teresa (et al.)

    Pages 45-57

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8961-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-8960-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Hepatic Stem Cells
Book Subtitle
Methods and Protocols
Editors
  • Naoki Tanimizu
Series Title
Methods in Molecular Biology
Series Volume
1905
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8961-4
DOI
10.1007/978-1-4939-8961-4
Hardcover ISBN
978-1-4939-8960-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 248
Number of Illustrations
11 b/w illustrations, 59 illustrations in colour
Topics