Methods in Molecular Biology

Human Monoclonal Antibodies

Methods and Protocols

Editors: Steinitz, Michael (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-8958-4
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About this book

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Unique and thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • Human Monoclonal Antibodies: The Benefits of Humanization

    Pages 1-10

    Waldmann, Herman

  • Cancer Immunotherapy: The Dawn of Antibody Cocktails

    Pages 11-51

    Marrocco, Ilaria (et al.)

  • IgM Natural Autoantibodies in Physiology and the Treatment of Disease

    Pages 53-81

    Fereidan-Esfahani, Mahboobeh (et al.)

  • Monoclonal Antibodies for the Treatment of Melanoma: Present and Future Strategies

    Pages 83-108

    Bhandaru, Madhuri (et al.)

  • An Efficient Method to Generate Monoclonal Antibodies from Human B Cells

    Pages 109-145

    Guthmiller, Jenna J. (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-4939-8958-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Human Monoclonal Antibodies
Book Subtitle
Methods and Protocols
Editors
  • Michael Steinitz
Series Title
Methods in Molecular Biology
Series Volume
1904
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8958-4
DOI
10.1007/978-1-4939-8958-4
Hardcover ISBN
978-1-4939-8957-7
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIV, 483
Number of Illustrations
20 b/w illustrations, 59 illustrations in colour
Topics