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Human Monoclonal Antibodies

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Michael Steinitz

    1. Department of Pathology, The Hebrew University, Hadassah Medical School, Jerusalem, Israel

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1904)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Human Monoclonal Antibodies: The Benefits of Humanization

    • Herman Waldmann
    Pages 1-10

  3. Cancer Immunotherapy: The Dawn of Antibody Cocktails

    • Ilaria Marrocco, Donatella Romaniello, Yosef Yarden
    Pages 11-51

  4. IgM Natural Autoantibodies in Physiology and the Treatment of Disease

    • Mahboobeh Fereidan-Esfahani, Tarek Nayfeh, Arthur Warrington, Charles L. Howe, Moses Rodriguez
    Pages 53-81

  5. Monoclonal Antibodies for the Treatment of Melanoma: Present and Future Strategies

    • Madhuri Bhandaru, Anand Rotte
    Pages 83-108

  6. An Efficient Method to Generate Monoclonal Antibodies from Human B Cells

    • Jenna J. Guthmiller, Haley L. Dugan, Karlynn E. Neu, Linda Yu-Ling Lan, Patrick C. Wilson
    Pages 109-145

  7. Isolation of Antigen-Specific, Antibody-Secreting Cells Using a Chip-Based Immunospot Array

    • Hiroyuki Kishi, Tatsuhiko Ozawa, Hiroshi Hamana, Eiji Kobayashi, Atsushi Muraguchi
    Pages 147-162

  8. Purification of Human Monoclonal Antibodies and Their Fragments

    • Nicole Ulmer, Sebastian Vogg, Thomas Müller-Späth, Massimo Morbidelli
    Pages 163-188

  9. One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral Blood

    • Anna-Jasmina Donaubauer, Paul F. Rühle, Ina Becker, Rainer Fietkau, Udo S. Gaipl, Benjamin Frey
    Pages 189-212

  10. Humanization and Simultaneous Optimization of Monoclonal Antibody

    • Taichi Kuramochi, Tomoyuki Igawa, Hiroyuki Tsunoda, Kunihiro Hattori
    Pages 213-230

  11. Antibody Fragments Humanization: Beginning with the End in Mind

    • Nicolas Aubrey, Philippe Billiald
    Pages 231-252

  12. Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice

    • Susana Magadán Mompó, África González-Fernández
    Pages 253-291

  13. Refining the Quality of Monoclonal Antibodies: Grafting Unique Peptide-Binding Site in the Fab Framework

    • Jeremy D. King, John C. Williams
    Pages 293-298

  14. Basic Procedures for Detection and Cytotoxicity of Chimeric Antigen Receptors

    • Keichiro Mihara, Tetsumi Yoshida, Joyeeta Bhattacharyya
    Pages 299-306

  15. Rapid Chimerization of Antibodies

    • Koji Hashimoto, Kohei Kurosawa, Hidetaka Seo, Kunihiro Ohta
    Pages 307-317

  16. Phage Display Technology for Human Monoclonal Antibodies

    • Marco Dal Ferro, Serena Rizzo, Emanuela Rizzo, Francesca Marano, Immacolata Luisi, Olga Tarasiuk et al.
    Pages 319-338

  17. Recombinant Antibody Selections by Combining Phage and Yeast Display

    • Fortunato Ferrara, Maria Felicia Soluri, Daniele Sblattero
    Pages 339-352

  18. Epitope Mapping via Phage Display from Single-Gene Libraries

    • Viola Fühner, Philip Alexander Heine, Kilian Johannes Carl Zilkens, Doris Meier, Kristian Daniel Ralph Roth, Gustavo Marçal Schmidt Garcia Moreira et al.
    Pages 353-375

  19. A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning

    • Angela Chiew Wen Ch’ng, Azimah Ahmad, Zoltán Konthur, Theam Soon Lim
    Pages 377-400

  20. A Binding Potency Assay for Pritumumab and Ecto-Domain Vimentin

    • Ivan Babic, Santosh Kesari, Mark C. Glassy
    Pages 401-415
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Keywords

About this book

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Uniqueand thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.


Editors and Affiliations

  • Department of Pathology, The Hebrew University, Hadassah Medical School, Jerusalem, Israel

    Michael Steinitz

Bibliographic Information

  • Book Title: Human Monoclonal Antibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Steinitz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8958-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8957-7Published: 12 December 2018

  • eBook ISBN: 978-1-4939-8958-4Published: 11 December 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 483

  • Number of Illustrations: 20 b/w illustrations, 59 illustrations in colour

  • Topics: Antibodies

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