- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Autoantibodies: Methods and Protocols aims to be helpful for all persons working with research and development of autoimmune laboratory diagnostics and for clinicians using autoantibody tests in daily work with patients.
- Table of contents (24 chapters)
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Autoantibodies as Diagnostic Tools
Pages 1-11
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Nonspecific Binding in Immunoassays for Autoantibodies
Pages 13-17
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Detection of Autoantibodies by Indirect Immunofluorescence Cytochemistry on Hep-2 Cells
Pages 19-46
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Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence
Pages 47-62
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Determination of Subset-Restricted Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Immunofluorescence Cytochemistry
Pages 63-77
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Table of contents (24 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Autoantibodies
- Book Subtitle
- Methods and Protocols
- Editors
-
- Gunnar Houen
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1901
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8949-2
- DOI
- 10.1007/978-1-4939-8949-2
- Hardcover ISBN
- 978-1-4939-8948-5
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 289
- Number of Illustrations
- 26 b/w illustrations, 32 illustrations in colour
- Topics