Barley

1. Front Matter

   Pages i-xi

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2. An Introduction to Barley: The Crop and the Model

   • Wendy A. Harwood

   Pages 1-5

3. Mutation Breeding in Barley: Historical Overview

   • Ludmila Ohnoutkova

   Pages 7-19

4. A Practical Guide to Barley Crossing

   • William T. B. Thomas, Hazel Bull, Allan Booth, Ruth Hamilton, Brian P. Forster, Jerome D. Franckowiak

   Pages 21-36

5. Barley Anther Culture

   • Ludmila Ohnoutkova, Tomas Vlcko, Mentewab Ayalew

   Pages 37-52

6. Isolated Microspore Culture in Barley

   • Patricio Esteves, François J. Belzile

   Pages 53-71

7. TILLING in Barley

   • Matthias Jost, Miriam Szurman-Zubrzycka, Katarzyna Gajek, Iwona Szarejko, Nils Stein

   Pages 73-94

8. Virus-Induced Gene Silencing (VIGS) for Functional Characterization of Disease Resistance Genes in Barley Seedlings

   • Lokanadha R. Gunupuru, Alexandre Perochon, Shahin S. Ali, Steven R. Scofield, Fiona M. Doohan

   Pages 95-114

9. Agrobacterium-Mediated Transformation of Barley Immature Embryos

   • Alison Hinchliffe, Wendy A. Harwood

   Pages 115-126

10. Methods for the Simple and Reliable Assessment of Barley Sensitivity to Abiotic Stresses During Early Development

    • Agata Daszkowska-Golec, Anna Skubacz, Marzena Kurowska, Michał Słota, Dorota Swiergolik, Iwona Szarejko

    Pages 127-151

11. Preparation of Barley Roots for Histological, Structural, and Immunolocalization Studies Using Light and Electron Microscopy

    • Marek Marzec, Michael Melzer

    Pages 153-166

12. Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy

    • Sybille Mittmann, Mikel Arrieta, Luke Ramsay, Robbie Waugh, Isabelle Colas

    Pages 167-179

13. Microarrays for High-Throughput Gene Expression Analysis of Barley

    • Jenny A. Morris, Pete E. Hedley

    Pages 181-194

14. Genome Engineering Using TALENs

    • Goetz Hensel, Jochen Kumlehn

    Pages 195-215

15. Creating Targeted Gene Knockouts in Barley Using CRISPR/Cas9

    • Tom Lawrenson, Wendy A. Harwood

    Pages 217-232

16. Genotyping-by-Sequencing on the Ion Torrent Platform in Barley

    • Amina Abed, Gaétan Légaré, Sonia Pomerleau, Jérôme St-Cyr, Brian Boyle, François J. Belzile

    Pages 233-252

17. DNA Methylation Analysis in Barley and Other Species with Large Genomes

    • Karolina Chwialkowska, Urszula Korotko, Miroslaw Kwasniewski

    Pages 253-268

18. High-Resolution RT-PCR Analysis of Alternative Barley Transcripts

    • Craig G. Simpson, John Fuller, Paulo Rapazote-Flores, Claus-Dieter Mayer, Cristiane P. G. Calixto, Linda Milne et al.

    Pages 269-281

19. Exome Capture for Variant Discovery and Analysis in Barley

    • Micha Bayer, Jenny A. Morris, Clare Booth, Allan Booth, Niki Uzrek, Joanne R. Russell et al.

    Pages 283-310

20. Back Matter

    Pages 311-313

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This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.

• Plant sciences
• Cereal genomics
• Crop genetics
• Mutation breeding
• Tissue culture
• Genotyping
• Gene expression

• Crop Transformation Group, Department of Crop Genetics, John Innes Centre, Norwich, UK

  Wendy A. Harwood

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