Methods in Molecular Biology

Bacteriophages

Methods and Protocols, Volume IV

Editors: Clokie, Martha R. J., Kropinski, Andrew, Lavigne, Rob (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-8940-9
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Hardcover $199.99
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About this book

This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. The chapters in this book cover molecular topics such as PhageFISH for monitoring phage infections at single cell level; the analysis of phage-host protein-protein interactions using Strep-tag® II purifications; and also application driven chapters including 'duckweed (Lemna minor) and alfalfa (Medicago sativa) as bacterial infection model systems’. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Innovative and thorough, Bacteriophages: Methods and Protocols, Volume IV is a valuable resource for both established and novice phage scientists.

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Table of contents (18 chapters)

Table of contents (18 chapters)
  • PhageFISH for Monitoring Phage Infections at Single Cell Level

    Barrero-Canosa, Jimena (et al.)

    Pages 1-26

  • Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria

    Piuri, Mariana (et al.)

    Pages 27-36

  • Engineering Bacteriophage-Based Biosensors

    Brownell, Daniel (et al.)

    Pages 37-50

  • Introduction of Phage Genome into Escherichia coli by Electroporation

    Janež, Nika (et al.)

    Pages 51-56

  • Site-Specific Mutagenesis of Bacillus subtilis Phage SPO1

    Stewart, Charles R.

    Pages 57-67

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8940-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-8939-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Bacteriophages
Book Subtitle
Methods and Protocols, Volume IV
Editors
  • Martha R. J. Clokie
  • Andrew Kropinski
  • Rob Lavigne
Series Title
Methods in Molecular Biology
Series Volume
1898
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8940-9
DOI
10.1007/978-1-4939-8940-9
Hardcover ISBN
978-1-4939-8939-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 216
Number of Illustrations
35 b/w illustrations, 46 illustrations in colour
Topics