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Bacteriophages

Methods and Protocols, Volume IV

  • Book
  • © 2019

Overview

Editors:
  1. Martha R. J. Clokie

    1. Department of Genetics and Genome Biology, University of Leicester, Leicester, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Andrew Kropinski

    1. Departments of Food Science, and Pathobiology, University of Guelph, Guelph, Canada

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    You can also search for this editor in PubMed   Google Scholar

  3. Rob Lavigne

    1. Laboratory of Gene Technology, KU Leuven, Leuven, Belgium

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1898)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. PhageFISH for Monitoring Phage Infections at Single Cell Level

    • Jimena Barrero-Canosa, Cristina Moraru
    Pages 1-26

  3. Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria

    • Mariana Piuri, Graham F. Hatfull
    Pages 27-36

  4. Engineering Bacteriophage-Based Biosensors

    • Daniel Brownell, John King, Brian Caliando, Lada Sycheva, Michael Koeris
    Pages 37-50

  5. Introduction of Phage Genome into Escherichia coli by Electroporation

    • Nika Janež, Saša Haberl Meglič, Karel Flisar, Damijan Miklavčič, Matjaž Peterka
    Pages 51-56

  6. Site-Specific Mutagenesis of Bacillus subtilis Phage SPO1

    • Charles R. Stewart
    Pages 57-67

  7. Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA

    • Laura J. Marinelli, Mariana Piuri, Graham F. Hatfull
    Pages 69-80

  8. Isolation of Competitive Phage Display-Modified Bacteriophage T4 with Affinity Chromatography

    • Krystyna Dąbrowska
    Pages 81-87

  9. Immobilization of Intact Phage and Phage-Derived Proteins for Detection and Biocontrol Purposes

    • Hany Anany, Luba Y. Brovko, Denis Arutyunov, Nilufar Poshtiban, Amit Singh, Upasana Singh et al.
    Pages 89-105

  10. Peptidoglycan Hydrolytic Activity of Bacteriophage Lytic Proteins in Zymogram Analysis

    • Lorena Rodríguez-Rubio, David M. Donovan, Beatriz Martínez, Ana Rodríguez, Pilar García
    Pages 107-115

  11. Analyzing Phage–Host Protein–Protein Interactions Using Strep-tag® II Purifications

    • Jeroen De Smet, Hanne Hendrix, An Van den Bossche
    Pages 117-136

  12. Techniques to Assess Phage–Biofilm Interaction

    • Diana Vilas Boas, Carina Almeida, Nuno Azevedo, Sanna Sillankorva, Joana Azeredo
    Pages 137-146

  13. Screening for Growth-Inhibitory ORFans in Pseudomonas aeruginosa-Infecting Bacteriophages

    • Hanne Hendrix, Ines Staes, Abram Aertsen, Jeroen Wagemans
    Pages 147-162

  14. Use of Greater Wax Moth Larvae (Galleria mellonella) as an Alternative Animal Infection Model for Analysis of Bacterial Pathogenesis

    • Fatima Kamal, Danielle L. Peters, Jaclyn G. McCutcheon, Gary B. Dunphy, Jonathan J. Dennis
    Pages 163-171

  15. Use of a Silkworm Larva Model in Phage Therapy Experiments

    • Jumpei Uchiyama, Iyo Takemura-Uchiyama, Shigenobu Matsuzaki
    Pages 173-181

  16. Exploitation of Drosophila Infection Models to Evaluate Antibacterial Efficacy of Phages

    • Hye-Jeong Jang, Hee-Won Bae, You-Hee Cho
    Pages 183-190

  17. Duckweed (Lemna minor) and Alfalfa (Medicago sativa) as Bacterial Infection Model Systems

    • Fatima Kamal, Alina Radziwon, Carly M. Davis, Jonathan J. Dennis
    Pages 191-198

  18. Use of a Chicken Embryo Lethality Assay to Assess the Efficacy of Phage Therapy

    • Angélina Trotereau, Catherine Schouler
    Pages 199-205

  19. Quantitating Phage Efficacy in Ready-To-Eat Meats

    • Andrew Chibeu, Sampathkumar Balamurugan
    Pages 207-214

  20. Back Matter

    Pages 215-216
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Keywords

About this book

This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. The chapters in this book cover molecular topics such as PhageFISH for monitoring phage infections at single cell level; the analysis of phage-host protein-protein interactions using Strep-tag® II purifications; and also application driven chapters including 'duckweed (Lemna minor) and alfalfa (Medicago sativa) as bacterial infection model systems’. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Innovative and thorough, Bacteriophages: Methods and Protocols, Volume IV is a valuable resource for both establishedand novice phage scientists.

Reviews

“The book describes the functional elucidation of bacteriophages in the current era, as well as the drive towards application-driven research. … this is a very interesting resource for protocols regarding phages and their use for therapy. I am going to include it in my reference material for my own students working on phages in relation to the gut microbiota.” (Koen Venema, Beneficial Microbes, Vol. 10 (3), 2019)

Editors and Affiliations

  • Department of Genetics and Genome Biology, University of Leicester, Leicester, UK

    Martha R. J. Clokie

  • Departments of Food Science, and Pathobiology, University of Guelph, Guelph, Canada

    Andrew Kropinski

  • Laboratory of Gene Technology, KU Leuven, Leuven, Belgium

    Rob Lavigne

About the editors

N/A

Bibliographic Information

  • Book Title: Bacteriophages

  • Book Subtitle: Methods and Protocols, Volume IV

  • Editors: Martha R. J. Clokie, Andrew Kropinski, Rob Lavigne

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8940-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8939-3Published: 20 December 2018

  • eBook ISBN: 978-1-4939-8940-9Published: 20 December 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 216

  • Number of Illustrations: 35 b/w illustrations, 46 illustrations in colour

  • Topics: Microbiology

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