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Nanotoxicity

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1894)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
  2. In Vitro Methods for Assessing Nanoparticle Toxicity

    • Dustin T. Savage, J. Zach Hilt, Thomas D. Dziubla
    Pages 1-29
  3. Deriving Neural Cells from Pluripotent Stem Cells for Nanotoxicity Testing

    • Yiling Hong, Nymph Chan, Aynun N. Begum
    Pages 57-72
  4. In Vitro Approaches for Assessing the Genotoxicity of Nanomaterials

    • Maria Dusinska, Espen Mariussen, Elise Rundén-Pran, Alexandra Misci Hudecova, Elisabeth Elje, Alena Kazimirova et al.
    Pages 83-122
  5. Application of Gelatin Zymography in Nanotoxicity Research

    • Yue Zhang, Rong Wan, Qunwei Zhang, Yiqun Mo
    Pages 133-143
  6. Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies

    • Rong Wan, Yiqun Mo, Ruirui Tong, Meiqin Gao, Qunwei Zhang
    Pages 145-159
  7. Analysis of Nanomaterial Toxicity by Western Blot

    • Gao Long, Yiqun Mo, Qunwei Zhang, Mizu Jiang
    Pages 161-169
  8. DNA Methylation Analysis

    • Lingfang Feng, Jianlin Lou
    Pages 181-227
  9. Evaluation of Nrf2 with Exposure to Nanoparticles

    • Fuli Zheng, Huangyuan Li
    Pages 229-246
  10. Application of Electron Microscopes in Nanotoxicity Assessment

    • Junzhe Zhang, Xiao He, Michael T. Tseng
    Pages 247-269
  11. Evaluation of Genotoxicity of Nanoparticles in Mouse Models

    • Sameera Nallanthighal, Ramune Reliene
    Pages 301-312
  12. Evaluation of Pulmonary Toxicity of Nanoparticles by Bronchoalveolar Lavage

    • Yiqun Mo, Yue Zhang, Qunwei Zhang
    Pages 313-322
  13. Pulmonary Function Testing in Animals

    • Gary W. Hoyle, Connie F. Schlueter, Sadiatu Musah
    Pages 323-330

About this book

This volume explores the latest techniques used to investigate nanotoxicity. The protocols covered in this book range from routine nanotoxicity assays to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver microtissue models; assessment of ovarian granulosa cells with exposure to nanoparticles; DNA methylation analysis; evaluation of genotoxicity of nanoparticles in mouse models; and studying nanotoxicity using embryonic zebrafish. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and practical, Nanotoxicity: Methods and Protocols is a valuable resource for researchers and postgraduate students in the fields of toxicology, environmental science, pharmacological sciences, and clinical medicine. This volume also serves as a starting point for researchers interested in learning more about this developing field.


Editors and Affiliations

  • Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, USA

    Qunwei Zhang

Bibliographic Information

  • Book Title: Nanotoxicity

  • Book Subtitle: Methods and Protocols

  • Editors: Qunwei Zhang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8916-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8915-7Published: 14 December 2018

  • eBook ISBN: 978-1-4939-8916-4Published: 13 December 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 371

  • Number of Illustrations: 16 b/w illustrations, 52 illustrations in colour

  • Topics: Pharmacology/Toxicology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access