- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key notes and advice from the experts
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- About this book
-
This detailed book covers numerous expert methodologies to examine Hippo signaling on the structural, molecular, cellular, and organismal level. Beginning with a section on Drosophila genetics, the volume continues with parts exploring molecular and cell biological studies of the Hippo pathway, the use of structural biology and biochemistry, and a brief look at the Hippo pathway in mouse models. Written for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, The Hippo Pathway: Methods and Protocols equips newcomers and specialists alike with key methodologies to accurately define the status of Hippo signaling in their experimental settings.
- Table of contents (26 chapters)
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The Power of Drosophila Genetics: The Discovery of the Hippo Pathway
Pages 3-26
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Drosophila Genetics: The Power of Genetic Mosaic Approaches
Pages 27-42
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Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues
Pages 43-51
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Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs
Pages 53-59
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Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence
Pages 61-73
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- The Hippo Pathway
- Book Subtitle
- Methods and Protocols
- Editors
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- Alexander Hergovich
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1893
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8910-2
- DOI
- 10.1007/978-1-4939-8910-2
- Hardcover ISBN
- 978-1-4939-8909-6
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIV, 374
- Number of Illustrations
- 15 b/w illustrations, 57 illustrations in colour
- Topics
