Methods in Molecular Biology

Myogenesis

Methods and Protocols

Editors: Ronning, Sissel Beate (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and advice from the experts
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eBook $169.00
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  • ISBN 978-1-4939-8897-6
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Hardcover $219.99
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About this book

This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS)

    Pakula, Anna (et al.)

    Pages 1-15

  • Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells

    Nikolić, Nataša (et al.)

    Pages 17-24

  • Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD

    Chen, Jingjuan (et al.)

    Pages 25-41

  • Chromatin Immunoprecipitation in Skeletal Myoblasts

    Rao, Vinay Kumar (et al.)

    Pages 43-54

  • Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor

    Turner, Daniel C. (et al.)

    Pages 55-79

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8897-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8896-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Myogenesis
Book Subtitle
Methods and Protocols
Editors
  • Sissel Beate Ronning
Series Title
Methods in Molecular Biology
Series Volume
1889
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8897-6
DOI
10.1007/978-1-4939-8897-6
Hardcover ISBN
978-1-4939-8896-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 351
Number of Illustrations
20 b/w illustrations, 64 illustrations in colour
Topics